| Literature DB >> 1383878 |
M Nakahiro1, O Arakawa, T Narahashi, S Ukai, Y Kato, K Nishinuma, T Nishimura.
Abstract
The effects of dimethyl sulfoxide (DMSO) on gamma-aminobutyric acid (GABA)-induced Cl- currents of rat dorsal root ganglion neurons in primary culture were studied by the whole-cell patch-clamp technique. Bath application of 5 microM GABA evoked sustained inward and outward currents at membrane potentials of -60 mV and +30 mV, respectively, when the external and internal solutions both contained 142 mM Cl-. DMSO at concentrations of 0.3-3% (v/v) caused a dose-dependent inhibition of the inward current induced by 5 microM GABA at -60 mV. DMSO at 3% inhibited the GABA-induced inward and outward currents at -60 mV and +30 mV to similar extents (54% and 53% of the control, respectively), but the time-course of inhibition of the outward current at +30 mV was much faster than that of the inward current at -60 mV. These results suggest that DMSO suppressed the currents by interacting with GABA receptor-Cl- channel complex protein rather than by affecting the lipid-bilayer of the cell membrane.Entities:
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Year: 1992 PMID： 1383878 DOI： 10.1016/0304-3940(92)90459-k
Source DB: PubMed Journal: Neurosci Lett ISSN： 0304-3940 Impact factor: 3.046