Literature DB >> 1382259

Use of a replica-plate assay for the rapid assessment of salivary protein-bacteria interactions.

C C Tseng1, F A Scannapieco, M J Levine.   

Abstract

A replica-plate assay was used to screen for the interaction of salivary molecules with dental plaque bacteria. Bacterial colonies cultured from supragingival plaque on sheep-blood (SB) agar were replica-plated onto nitrocellulose membranes overlaying SB or mitis-salivarius agar. Membranes with attached colonies were removed and incubated with 125I-amylase or 125I-proline-rich glycoprotein (PRG). Positive interactions were detected by autoradiography. Only strains of Streptococcus gordonii and Actinomyces viscosus bound amylase, and strains of A. viscosus bound PRG. The results suggest that amylase and PRG bind to selected species of aerobic dental plaque bacteria.

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Year:  1992        PMID： 1382259     DOI： 10.1111/j.1399-302x.1992.tb00021.x

Source DB:  PubMed          Journal:  Oral Microbiol Immunol        ISSN： 0902-0055


  5 in total

1.  Role of Streptococcus gordonii amylase-binding protein A in adhesion to hydroxyapatite, starch metabolism, and biofilm formation.

Authors:  J D Rogers; R J Palmer; P E Kolenbrander; F A Scannapieco
Journal:  Infect Immun       Date:  2001-11       Impact factor: 3.441

Review 2.  Taking the starch out of oral biofilm formation: molecular basis and functional significance of salivary α-amylase binding to oral streptococci.

Authors:  Anna E Nikitkova; Elaine M Haase; Frank A Scannapieco
Journal:  Appl Environ Microbiol       Date:  2012-11-09       Impact factor: 4.792

3.  Prevalence of the amylase-binding protein A gene (abpA) in oral streptococci.

Authors:  A E Brown; J D Rogers; E M Haase; P M Zelasko; F A Scannapieco
Journal:  J Clin Microbiol       Date:  1999-12       Impact factor: 5.948

4.  Probing the role of aromatic residues at the secondary saccharide-binding sites of human salivary alpha-amylase in substrate hydrolysis and bacterial binding.

Authors:  Chandran Ragunath; Suba G A Manuel; Venkat Venkataraman; Hameetha B R Sait; Chinnasamy Kasinathan; Narayanan Ramasubbu
Journal:  J Mol Biol       Date:  2008-10-14       Impact factor: 5.469

5.  Characterization of an amylase-binding component of Streptococcus gordonii G9B.

Authors:  F A Scannapieco; G G Haraszthy; M I Cho; M J Levine
Journal:  Infect Immun       Date:  1992-11       Impact factor: 3.441
  5 in total

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