| Literature DB >> 1381647 |
Abstract
A Trichophyton schoenleinii (T. schoenleinii) strain from tinea favus was cultured in a liquid medium, from which an extracellular keratinase extract was obtained. The keratinase was partially purified with carboxymethyl-cellulose (CMC) column chromatography. Some biochemical characteristics of the keratinase were then examined. Its molecular weight was estimated to be 38,000 on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The optimal temperature was 50 degrees C, and the optimal pH value was 5.5. The keratinolytic activity was specifically increased by Fe++ and Fe .Entities:
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Year: 1992 PMID： 1381647 DOI： 10.1016/s0934-8840(11)80618-3
Source DB: PubMed Journal: Zentralbl Bakteriol ISSN： 0934-8840