Literature DB >> 1380250

A simple procedure for recovering the denaturing effect of methylmercury in agarose gel electrophoresis.

P Fernández-Silva1, J A Enriquez, J Montoya.   

Abstract

A simple and rapid procedure for recovering the denaturing effect of methylmercuric hydroxide in agarose gel electrophoresis is described. The procedure consisted of the treatment of the commercial methylmercuric hydroxide solutions with Amberlite, a mixture of anion- and cation-exchange resins. This treatment greatly improved the resolution of RNA species when fractionated by electrophoresis through agarose-CH3HgOH slab gel.

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Year:  1992        PMID： 1380250

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  5 in total

1.  Transient activation of mitochondrial translation regulates the expression of the mitochondrial genome during mammalian mitochondrial differentiation.

Authors:  L K Ostronoff; J M Izquierdo; J A Enríquez; J Montoya; J M Cuezva
Journal:  Biochem J       Date:  1996-05-15       Impact factor: 3.857

2.  Direct regulation of mitochondrial RNA synthesis by thyroid hormone.

Authors:  J A Enríquez; P Fernández-Silva; N Garrido-Pérez; M J López-Pérez; A Pérez-Martos; J Montoya
Journal:  Mol Cell Biol       Date:  1999-01       Impact factor: 4.272

3.  Analysis of the transcription products of the rainbow trout (Oncorynchus mykiss) liver mitochondrial genome: detection of novel mitochondrial transcripts.

Authors:  R Zardoya; A Pérez-Martos; J M Bautista; J Montoya
Journal:  Curr Genet       Date:  1995-06       Impact factor: 3.886

4.  A variant form of the nuclear triiodothyronine receptor c-ErbAalpha1 plays a direct role in regulation of mitochondrial RNA synthesis.

Authors:  F Casas; P Rochard; A Rodier; I Cassar-Malek; S Marchal-Victorion; R J Wiesner; G Cabello; C Wrutniak
Journal:  Mol Cell Biol       Date:  1999-12       Impact factor: 4.272

5.  ATP synthesis is coupled to rat liver mitochondrial RNA synthesis.

Authors:  S F DasGupta; S I Rapoport; M Gerschenson; E Murphy; G Fiskum; S J Russell; K Chandrasekaran
Journal:  Mol Cell Biochem       Date:  2001-05       Impact factor: 3.396
  5 in total

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