Literature DB >> 1379606

Direct sequencing of large flavivirus PCR products for analysis of genome variation and molecular epidemiological investigations.

J G Lewis1, G J Chang, R S Lanciotti, D W Trent.   

Abstract

The polymerase chain reaction (PCR) was used to amplify viral cDNAs from selected regions of dengue genomic RNA by using appropriate 'consensus' primers. DNA amplicons containing the structural genes from all 4 dengue serotypes were prepared and directly sequenced using dengue-virus-specific primers. This method can characterize reliably flavivirus field isolates at the molecular level without extensive virus propagation and molecular cloning, and will be a valuable tool for molecular epidemiological studies.

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Year:  1992        PMID： 1379606     DOI： 10.1016/0166-0934(92)90165-a

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  17 in total

1.  Development of reverse transcription-PCR assays specific for detection of equine encephalitis viruses.

Authors:  B Linssen; R M Kinney; P Aguilar; K L Russell; D M Watts; O R Kaaden; M Pfeffer
Journal:  J Clin Microbiol       Date:  2000-04       Impact factor: 5.948

2.  Evolutionary relationships of endemic/epidemic and sylvatic dengue viruses.

Authors:  E Wang; H Ni; R Xu; A D Barrett; S J Watowich; D J Gubler; S C Weaver
Journal:  J Virol       Date:  2000-04       Impact factor: 5.103

3.  Chimeric dengue type 2 (vaccine strain PDK-53)/dengue type 1 virus as a potential candidate dengue type 1 virus vaccine.

Authors:  C Y Huang; S Butrapet; D J Pierro; G J Chang; A R Hunt; N Bhamarapravati; D J Gubler; R M Kinney
Journal:  J Virol       Date:  2000-04       Impact factor: 5.103

Review 4.  Advances in dengue diagnosis.

Authors:  M G Guzmán; G Kourí
Journal:  Clin Diagn Lab Immunol       Date:  1996-11

5.  Clostridium perfringens bacteriophages ΦCP39O and ΦCP26F: genomic organization and proteomic analysis of the virions.

Authors:  Bruce S Seal; Derrick E Fouts; Mustafa Simmons; Johnna K Garrish; Robin L Kuntz; Rebekah Woolsey; Kathleen M Schegg; Andrew M Kropinski; Hans-W Ackermann; Gregory R Siragusa
Journal:  Arch Virol       Date:  2010-10-21       Impact factor: 2.574

6.  Use of a heteroduplex mobility assay to detect differences in the fusion protein cleavage site coding sequence among Newcastle disease virus isolates.

Authors:  A Berinstein; H S Sellers; D J King; B S Seal
Journal:  J Clin Microbiol       Date:  2001-09       Impact factor: 5.948

7.  Detection of dengue virus RNA in patients after primary or secondary dengue infection by using the TaqMan automated amplification system.

Authors:  T Laue; P Emmerich; H Schmitz
Journal:  J Clin Microbiol       Date:  1999-08       Impact factor: 5.948

8.  Recombinant, live-attenuated tetravalent dengue virus vaccine formulations induce a balanced, broad, and protective neutralizing antibody response against each of the four serotypes in rhesus monkeys.

Authors:  Joseph E Blaney; Jennifer M Matro; Brian R Murphy; Stephen S Whitehead
Journal:  J Virol       Date:  2005-05       Impact factor: 5.103

9.  Nucleotide and predicted amino acid sequence analysis of the fusion protein and hemagglutinin-neuraminidase protein genes among Newcastle disease virus isolates. Phylogenetic relationships among the Paramyxovirinae based on attachment glycoprotein sequences.

Authors:  Bruce S Seal
Journal:  Funct Integr Genomics       Date:  2004-04-24       Impact factor: 3.410

10.  Analysis of matrix protein gene nucleotide sequence diversity among Newcastle disease virus isolates demonstrates that recent disease outbreaks are caused by viruses of psittacine origin.

Authors:  B S Seal
Journal:  Virus Genes       Date:  1995       Impact factor: 2.332
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