Literature DB >> 137900

Purification and properties of the P15 specific restriction endonuclease from Escherichia coli.

J Reiser, R Yuan.   

Abstract

The specific restriction endonuclease of the Escherichia coli plasmid, P15, has been purified to apparent homogeneity by a procedure that includes DNA-cellulose chromatography as well as a new endonuclease assay. Sedimentation on glycerol gradients showed two peaks of activity with values of 11.3 S and 15.7 S. The highly purified enzyme requires ATP and Mg2+ for activity and is stimulated by S-adenosylmethionine. A methylase activity is observed in the course of the endonucleolytic reaction which protects some of the DNA sites from cleavage.

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Year:  1977        PMID： 137900

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  17 in total

Review 1.  Nucleoside triphosphate-dependent restriction enzymes.

Authors:  D T Dryden; N E Murray; D N Rao
Journal:  Nucleic Acids Res       Date:  2001-09-15       Impact factor: 16.971

2.  Unidirectional translocation from recognition site and a necessary interaction with DNA end for cleavage by Type III restriction enzyme.

Authors:  Nidhanapati K Raghavendra; Desirazu N Rao
Journal:  Nucleic Acids Res       Date:  2004-10-22       Impact factor: 16.971

3.  Structural insights into the assembly and shape of Type III restriction-modification (R-M) EcoP15I complex by small-angle X-ray scattering.

Authors:  Yogesh K Gupta; Lin Yang; Siu-Hong Chan; James C Samuelson; Shuang-yong Xu; Aneel K Aggarwal
Journal:  J Mol Biol       Date:  2012-05-02       Impact factor: 5.469

Review 4.  The phasevarion: phase variation of type III DNA methyltransferases controls coordinated switching in multiple genes.

Authors:  Yogitha N Srikhanta; Kate L Fox; Michael P Jennings
Journal:  Nat Rev Microbiol       Date:  2010-02-08       Impact factor: 60.633

5.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1990-04-25       Impact factor: 16.971

6.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1989       Impact factor: 16.971

7.  Restriction and modification enzymes and their recognition sequences.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1985       Impact factor: 16.971

8.  Characterization of mutations of the bacteriophage P1 mod gene encoding the recognition subunit of the EcoP1 restriction and modification system.

Authors:  D N Rao; H Eberle; T A Bickle
Journal:  J Bacteriol       Date:  1989-05       Impact factor: 3.490

9.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1988       Impact factor: 16.971

10.  Characterization of BseMII, a new type IV restriction-modification system, which recognizes the pentanucleotide sequence 5'-CTCAG(N)(10/8)/.

Authors:  S Jurenaite-Urbanaviciene; R Kazlauskiene; V Urbelyte; Z Maneliene; M Petrusyte; A Lubys; A Janulaitis
Journal:  Nucleic Acids Res       Date:  2001-02-15       Impact factor: 16.971
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