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Literature DB >> 1378617

Identification of Tyr-185 as the site of tyrosine autophosphorylation of recombinant mitogen-activated protein kinase p42mapk.

A J Rossomando¹, J Wu, H Michel, J Shabanowitz, D F Hunt, M J Weber, T W Sturgill.

Abstract

Tyrosine phosphorylation of 42-kDa mitogen-activated protein kinase (p42mapk) occurs during expression of the recombinant protein in Escherichia coli, as well as during in vitro phosphorylation of the protein purified from this source. Structural analyses were performed to identify the site(s) of tyrosine phosphorylation of recombinant p42mapk, both during expression of the protein in E. coli and during in vitro incubations with ATP/Mg2+/Mn2+. Mass spectrometry and phosphopeptide mapping showed that tyrosine phosphorylation of recombinant p42mapk occurs on Tyr-185, the site of regulatory tyrosine phosphorylation that occurs in mitogen-stimulated mammalian cells.

Entities: Chemical Gene Species

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Year: 1992 PMID： 1378617 PMCID： PMC402101 DOI： 10.1073/pnas.89.13.5779

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

27 in total

1. ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF.

Authors: T G Boulton; S H Nye; D J Robbins; N Y Ip; E Radziejewska; S D Morgenbesser; R A DePinho; N Panayotatos; M H Cobb; G D Yancopoulos
Journal: Cell Date: 1991-05-17 Impact factor: 41.582

2. Sequence of pp42/MAP kinase, a serine/threonine kinase regulated by tyrosine phosphorylation.

Authors: J H Her; J Wu; T B Rall; T W Sturgill; M J Weber
Journal: Nucleic Acids Res Date: 1991-07-11 Impact factor: 16.971

3. Autophosphorylation in vitro of recombinant 42-kilodalton mitogen-activated protein kinase on tyrosine.

Authors: J Wu; A J Rossomando; J H Her; R Del Vecchio; M J Weber; T W Sturgill
Journal: Proc Natl Acad Sci U S A Date: 1991-11-01 Impact factor: 11.205

4. Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase.

Authors: D R Knighton; J H Zheng; L F Ten Eyck; N H Xuong; S S Taylor; J M Sowadski
Journal: Science Date: 1991-07-26 Impact factor: 47.728

5. Tyrosine and threonine phosphorylation of an immunoaffinity-purified 44-kDa MAP kinase.

Authors: J C Scimeca; R Ballotti; T T Nguyen; C Filloux; E Van Obberghen
Journal: Biochemistry Date: 1991-09-24 Impact factor: 3.162

6. Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinase.

Authors: N G Anderson; J L Maller; N K Tonks; T W Sturgill
Journal: Nature Date: 1990-02-15 Impact factor: 49.962

7. Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.

Authors: J Posada; J Sanghera; S Pelech; R Aebersold; J A Cooper
Journal: Mol Cell Biol Date: 1991-05 Impact factor: 4.272

8. Identification by mass spectrometry of threonine 97 in bovine myelin basic protein as a specific phosphorylation site for mitogen-activated protein kinase.

Authors: A K Erickson; D M Payne; P A Martino; A J Rossomando; J Shabanowitz; M J Weber; D F Hunt; T W Sturgill
Journal: J Biol Chem Date: 1990-11-15 Impact factor: 5.157

9. Multiple components in an epidermal growth factor-stimulated protein kinase cascade. In vitro activation of a myelin basic protein/microtubule-associated protein 2 kinase.

Authors: N G Ahn; R Seger; R L Bratlien; C D Diltz; N K Tonks; E G Krebs
Journal: J Biol Chem Date: 1991-03-05 Impact factor: 5.157

10. Requirements for phosphorylation of MAP kinase during meiosis in Xenopus oocytes.

Authors: J Posada; J A Cooper
Journal: Science Date: 1992-01-10 Impact factor: 47.728

21 in total

1. Positive and negative regulation of Raf kinase activity and function by phosphorylation.

Authors: H Chong; J Lee; K L Guan
Journal: EMBO J Date: 2001-07-16 Impact factor: 11.598

2. Selective detection of phosphopeptides in complex mixtures by electrospray liquid chromatography/mass spectrometry.

Authors: M J Huddleston; R S Annan; M F Bean; S A Carr
Journal: J Am Soc Mass Spectrom Date: 1993-09 Impact factor: 3.109

3. Identification of phosphorylation sites in phosphopeptides by positive and negative mode electrospray ionization-tandem mass spectrometry.

Authors: M Busman; K L Schey; J E Oatis; D R Knapp
Journal: J Am Soc Mass Spectrom Date: 1996-03 Impact factor: 3.109

Identification of Tyr-185 as the site of tyrosine autophosphorylation of recombinant mitogen-activated protein kinase p42mapk.

1. ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF.

2. Sequence of pp42/MAP kinase, a serine/threonine kinase regulated by tyrosine phosphorylation.

3. Autophosphorylation in vitro of recombinant 42-kilodalton mitogen-activated protein kinase on tyrosine.

4. Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase.

5. Tyrosine and threonine phosphorylation of an immunoaffinity-purified 44-kDa MAP kinase.

6. Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinase.

7. Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.

8. Identification by mass spectrometry of threonine 97 in bovine myelin basic protein as a specific phosphorylation site for mitogen-activated protein kinase.

9. Multiple components in an epidermal growth factor-stimulated protein kinase cascade. In vitro activation of a myelin basic protein/microtubule-associated protein 2 kinase.

10. Requirements for phosphorylation of MAP kinase during meiosis in Xenopus oocytes.

1. Positive and negative regulation of Raf kinase activity and function by phosphorylation.

2. Selective detection of phosphopeptides in complex mixtures by electrospray liquid chromatography/mass spectrometry.

3. Identification of phosphorylation sites in phosphopeptides by positive and negative mode electrospray ionization-tandem mass spectrometry.

4. Active ERK2 is sufficient to mediate growth arrest and differentiation signaling.

5. Plasmodium falciparum possesses a unique dual-specificity serine/threonine and tyrosine kinase, Pfnek3.

6. Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1.

7. RNA Recognition-like Motifs Activate a Mitogen-Activated Protein Kinase.

8. Growth arrest signaling of the Raf/MEK/ERK pathway in cancer.

9. A role for autophosphorylation revealed by activated alleles of FUS3, the yeast MAP kinase homolog.

10. Activation of the Smk1 mitogen-activated protein kinase by developmentally regulated autophosphorylation.