Literature DB >> 13785

Validation of a simple radiochemical assay measuring hydrolysis of choline-labelled microsomal phosphatidylcholine by phospholipase C. pH-dependence.

B R Cater, P Trivedi, T Hallinan.   

Abstract

Selective hydrolysis of phosphatidylcholine species, which are selectively radioactively labelled in vivo, does not appear to interfere with a radiochemical assay for hydrolysis of microsomal phosphatidylcholine by C-type phospholipases from Bacillus cereus or Clostridium perfringens. Both phospholipases substantially hydrolysed phosphatidylcholine over the pH range 4.0-10.0.

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Year:  1976        PMID： 13785      PMCID： PMC1164263          DOI： 10.1042/bj1600475

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  23 in total

1.  Effect of Clostridium perfringens phospholipase C(alpha-toxin) on the human diploid fibroblast membrane.

Authors:  R Möllby; M Thelestam; T Wadström
Journal:  J Membr Biol       Date:  1974       Impact factor: 1.843

2.  Biological activities contaminating preparations of phospholipase C ( -toxin) from Clostridium perfringens.

Authors:  R Möllby; C E Nord; T Wadström
Journal:  Toxicon       Date:  1973-02       Impact factor: 3.033

3.  Acetanilide-hydrolyzing esterase of rat liver microsomes. I. Solubilization, purification, and intramicrosomal localization.

Authors:  T Akao; T Omura
Journal:  J Biochem       Date:  1972-11       Impact factor: 3.387

4.  The selective loss of lysophospholipids in some commonly used lipid-extraction procedures.

Authors:  K S Bjerve; L N Daae; J Bremer
Journal:  Anal Biochem       Date:  1974-03       Impact factor: 3.365

5.  Quantitative studies on the preservation of choline and ethanolamine phosphatides during tissue preparation for electron microscopy. I. Glutaraldehyde, osmium tetroxide, Araldite methods.

Authors:  G H Cope; M A Williams
Journal:  J Microsc       Date:  1969       Impact factor: 1.758

6.  The use of radioactive choline as a label for microsomal membranes. I. Selectivity of label for endoplasmic reticulum and specificity for lecithin.

Authors:  P Nagley; T Hallinan
Journal:  Biochim Biophys Acta       Date:  1968-09-17

7.  Phospholipase C from Bacillus cereus and its use in studies of tissue thromboplastin.

Authors:  A B Otnaess; H Prydz; E Bjorklid; A Berre
Journal:  Eur J Biochem       Date:  1972-05-23

8.  Phospholipase activity in rat-liver microsomes studied by the use of endogenous substrates.

Authors:  P Bjornstad
Journal:  Biochim Biophys Acta       Date:  1966-06-01

9.  Guinea pig liver microsomal UDP-glucuronyltransferase: compartmented or phospholipid-constrained?

Authors:  C Berry; A Stellon; T Hallinan
Journal:  Biochim Biophys Acta       Date:  1975-10-22

10.  Phenobarbital-induced alterations in phosphatidylcholine and triglyceride synthesis in hepatic endoplasmic reticulum.

Authors:  D L Young; G Powell; W O McMillan
Journal:  J Lipid Res       Date:  1971-01       Impact factor: 5.922
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  1 in total

1.  Phospholipid content and activity of pure uridine diphosphate-glucuronyltransferase from rat liver.

Authors:  B Burchell; T Hallinan
Journal:  Biochem J       Date:  1978-06-01       Impact factor: 3.857
  1 in total

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