Literature DB >> 1378362

Monoclonal antibody epitopes of mycobacterial 65-kD heat-shock protein defined by epitope scanning.

A H Hajeer1, J Worthington, K Morgan, R M Bernstein.   

Abstract

The binding sites for MoAbs to the 65-kD heat-shock protein (hsp65) of mycobacteria have been investigated by epitope scanning. Five hundred and twenty-six 8-mer peptides representing the complete sequence of Mycobacterium tuberculosis hsp65 were synthesised in duplicate using the Epitope Scanning Kit (CRB Ltd.). The epitopes of six MoAbs raised to the hsp65 of M. tuberculosis or M. leprae were investigated. We have identified the epitope of a new MoAb (DC16); this epitope is continuous, hydrophilic in nature and 11 amino acids long. We have also confirmed the location of the epitopes of three MoAbs (IIH9, ML30 and IIC8). Thus the epitope scanning technique has proved suitable for the detection of continuous epitopes of hsp65.

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Year:  1992        PMID： 1378362      PMCID： PMC1554412          DOI： 10.1111/j.1365-2249.1992.tb06888.x

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN： 0009-9104            Impact factor:   4.330


  17 in total

1.  Antibody to a 63 kilodalton insect protein in ankylosing spondylitis.

Authors:  S R Brand; J Worthington; D P McIntosh; R M Bernstein
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2.  Identification of antibody epitopes within the CB-11 peptide of type II collagen. I: Detection of antibody binding sites by epitope scanning.

Authors:  J Worthington; A Brass; K Morgan
Journal:  Autoimmunity       Date:  1991       Impact factor: 2.815

3.  T lymphocyte clones illuminate pathogenesis and affect therapy of experimental arthritis.

Authors:  I R Cohen; J Holoshitz; W van Eden; A Frenkel
Journal:  Arthritis Rheum       Date:  1985-08

4.  Antibody levels to mycobacteria in relation to HLA type: evidence for non-HLA-linked high levels of antibody to the 65 kD heat shock protein of M. bovis in rheumatoid arthritis.

Authors:  G M Bahr; G A Rook; M al-Saffar; J Van Embden; J L Stanford; K Behbehani
Journal:  Clin Exp Immunol       Date:  1988-11       Impact factor: 4.330

5.  Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid.

Authors:  H M Geysen; R H Meloen; S J Barteling
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205

6.  A simple method for displaying the hydropathic character of a protein.

Authors:  J Kyte; R F Doolittle
Journal:  J Mol Biol       Date:  1982-05-05       Impact factor: 5.469

7.  A monoclonal antibody to the mycobacterial 65 kDa heat shock protein (ML 30) binds to cells in normal and arthritic joints of rats.

Authors:  S Kleinau; K Söderström; R Kiessling; L Klareskog
Journal:  Scand J Immunol       Date:  1991-02       Impact factor: 3.487

8.  Identification of a novel B-cell epitope of restricted specificity on the hsp 65-kDa protein of Mycobacterium tuberculosis.

Authors:  A Rambukkana; S Yong; P K Das
Journal:  FEMS Microbiol Immunol       Date:  1991-02

9.  Characterization of antibody-reactive epitopes on the 65-kilodalton protein of Mycobacterium leprae.

Authors:  T M Buchanan; H Nomaguchi; D C Anderson; R A Young; T P Gillis; W J Britton; J Ivanyi; A H Kolk; O Closs; B R Bloom
Journal:  Infect Immun       Date:  1987-04       Impact factor: 3.441

10.  Antibodies that are specific for a single amino acid interchange in a protein epitope use structurally distinct variable regions.

Authors:  S E Stark; A J Caton
Journal:  J Exp Med       Date:  1991-09-01       Impact factor: 14.307
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  3 in total

1.  Marked enhancement of the antigen-specific immune response by combining plasmid DNA-based immunization with a Schiff base-forming drug.

Authors:  Jehad Charo; Maria Sundbäck; Ken Wasserman; Anne-Marie T Ciupitu; Babak Mirzai; Ruurd van der Zee; Rolf Kiessling
Journal:  Infect Immun       Date:  2002-12       Impact factor: 3.441

2.  Antibodies to major histocompatibility complex class II inhibit proliferation, but increase production of soluble CD23 in lymphoblastoid B-cell lines.

Authors:  A H Hajeer; N Snowden; P Wilson; S Drover; W E Ollier
Journal:  Immunology       Date:  1993-12       Impact factor: 7.397

Review 3.  The antigen 85 complex: a major secretion product of Mycobacterium tuberculosis.

Authors:  H G Wiker; M Harboe
Journal:  Microbiol Rev       Date:  1992-12
  3 in total

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