Literature DB >> 1377374

Fractionation and antigenic characterization of organelles of Eimeria tenella sporozoites.

U Kawazoe1, F M Tomley, J A Frazier.   

Abstract

Sporozoites of Eimeria tenella were disrupted by sonication and subcellular fractions were separated by sucrose gradient ultracentrifugation. Fractions from gradients were characterized by electron microscopical appearance and their polypeptide and antigenic profiles determined by PAGE and Western blotting with antisera to sporozoites and 1st- and 2nd-generation merozoites. Fractions containing micronemes, rhoptries or membranes showed markedly different polypeptide content and antigenic reactivity. Microneme epitopes were strongly conserved between sporozoites and 2nd-generation merozoites whereas the majority of rhoptry epitopes and many membrane epitopes were sporozoite specific. The only polypeptide of sporozoites which was strongly recognized by antisera raised to 1st generation merozoites was a microneme antigen of molecular weight approximately 100 kDa.

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Year:  1992        PMID： 1377374     DOI： 10.1017/s003118200006073x

Source DB:  PubMed          Journal:  Parasitology        ISSN： 0031-1820            Impact factor:   3.234


  9 in total

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Authors:  M Tilley; M E Fichera; M E Jerome; D S Roos; M W White
Journal:  Infect Immun       Date:  1997-11       Impact factor: 3.441

2.  Nitrocellulose immunoblotting for identification and molecular gene cloning of Eimeria maxima antigens that stimulate lymphocyte proliferation.

Authors:  J M Bumstead; P P Dunn; F M Tomley
Journal:  Clin Diagn Lab Immunol       Date:  1995-09

3.  Characterisation of Plasmodium invasive organelles; an ookinete microneme proteome.

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Journal:  Proteomics       Date:  2009-03       Impact factor: 3.984

4.  Preparation and initial application of monoclonal antibodies that recognize Eimeria tenella microneme proteins 1 and 2.

Authors:  Qing Liu; Zhengtao Chen; Wenyan Shi; Hui Sun; Jie Zhang; Hongmei Li; Yihong Xiao; Fangkun Wang; Xiaomin Zhao
Journal:  Parasitol Res       Date:  2014-08-28       Impact factor: 2.289

5.  Proteomic comparison of four Eimeria tenella life-cycle stages: unsporulated oocyst, sporulated oocyst, sporozoite and second-generation merozoite.

Authors:  Kalpana Lal; Elizabeth Bromley; Richard Oakes; Judith Helena Prieto; Sanya J Sanderson; Dominic Kurian; Lawrence Hunt; John R Yates; Jonathan M Wastling; Robert E Sinden; Fiona M Tomley
Journal:  Proteomics       Date:  2009-10       Impact factor: 3.984

6.  Characterization of rhoptry proteins of Eimeria tenella sporozoites: antigenic diversity of rhoptry epitopes within species of the genus Eimeria and among three asexual generations of a single species, E. tenella.

Authors:  F M Tomley
Journal:  Infect Immun       Date:  1994-10       Impact factor: 3.441

7.  Electrical cream separator coupled with vacuum filtration for the purification of eimerian oocysts and trichostrongylid eggs.

Authors:  Saeed El-Ashram; Xun Suo
Journal:  Sci Rep       Date:  2017-02-24       Impact factor: 4.379

8.  Protective Immunity Induced by an Eimeria tenella Whole Sporozoite Vaccine Elicits Specific B-Cell Antigens.

Authors:  Marco A Juárez-Estrada; Amanda Gayosso-Vázquez; Guillermo Tellez-Isaias; Rogelio A Alonso-Morales
Journal:  Animals (Basel)       Date:  2021-05-09       Impact factor: 2.752

9.  A rapid and simple single-step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites.

Authors:  Saeed El-Ashram; Yu Zhang; Yongsheng Ji; Dina Salama; Kun Mei; Li Zhili; Huang Shujian; Haoji Zhang; Shawky M Aboelhadid; Reem A Alajmi; Dina M Metwally; Manal F El-Khadragy; Billy M Hargis; Guillermo Tellez-Isaias; Beniamino T Cenci-Goga; Musafiri Karama; Munyaradzi C Marufu; Fathi Abouhajer; Gamal Ali Abdelhafez Hamady; Abeer El Wakil; Ibrahim Al Nasr; Xun Suo
Journal:  Vet Med Sci       Date:  2020-09-26
  9 in total

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