Literature DB >> 1377081

A prominent epitope on GABAA receptors is recognized by two different monoclonal antibodies.

M Ewert1, A L de Blas, H Möhler, P H Seeburg.   

Abstract

The monoclonal antibody 62-3G1 raised against the GABAA/benzodiazepine receptor complex was tested for its subunit selectivity using recombinantly expressed GABAA receptor subunits. The antibody bound selectively to beta 2 and beta 3 but not beta 1 nor any other GABAA receptor subunit. Using enzyme-linked immunosorbent assay, the epitope on beta 2 and beta 3 subunits was determined to be residues 1-3. MAb bd 17, which displays identical subunit selectivity as mAb 62-3G1, was seen to bind to the same epitope. These results resolve the subunit selectivity of mAbs 62-3G1 and bd 17 and reveal the identity and localization of a prominent immunogenic epitope on the GABAA/benzodiazepine receptor.

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Year:  1992        PMID： 1377081     DOI： 10.1016/0006-8993(92)90368-j

Source DB:  PubMed          Journal:  Brain Res        ISSN： 0006-8993            Impact factor:   3.252


  18 in total

1.  GABAergic innervation organizes synaptic and extrasynaptic GABAA receptor clustering in cultured hippocampal neurons.

Authors:  Sean B Christie; Celia P Miralles; Angel L De Blas
Journal:  J Neurosci       Date:  2002-02-01       Impact factor: 6.167

2.  Synaptic and nonsynaptic localization of protocadherin-gammaC5 in the rat brain.

Authors:  Yanfang Li; David R Serwanski; Celia P Miralles; Christopher G Fiondella; Joseph J Loturco; Maria E Rubio; Angel L De Blas
Journal:  J Comp Neurol       Date:  2010-09-01       Impact factor: 3.215

3.  Neuregulin induces GABA(A) receptor subunit expression and neurite outgrowth in cerebellar granule cells.

Authors:  H I Rieff; L T Raetzman; D W Sapp; H H Yeh; R E Siegel; G Corfas
Journal:  J Neurosci       Date:  1999-12-15       Impact factor: 6.167

4.  Synaptic and nonsynaptic localization of GABAA receptors containing the alpha5 subunit in the rat brain.

Authors:  David R Serwanski; Celia P Miralles; Sean B Christie; Ashok K Mehta; Xuejing Li; Angel L De Blas
Journal:  J Comp Neurol       Date:  2006-11-20       Impact factor: 3.215

Review 5.  Brain GABAA receptors studied with subunit-specific antibodies.

Authors:  A L De Blas
Journal:  Mol Neurobiol       Date:  1996-02       Impact factor: 5.590

6.  Immunocytochemical localization of the GABAc receptor rho subunits in the mammalian retina.

Authors:  R Enz; J H Brandstätter; H Wässle; J Bormann
Journal:  J Neurosci       Date:  1996-07-15       Impact factor: 6.167

7.  Localization of GABAA receptors in the rabbit retina.

Authors:  U Greferath; U Grünert; F Müller; H Wässle
Journal:  Cell Tissue Res       Date:  1994-05       Impact factor: 5.249

8.  Effects of cell signaling on the development of GABA receptors in chick retina neurons.

Authors:  B H Shah; R E Hausman
Journal:  Neurochem Res       Date:  1993-09       Impact factor: 3.996

9.  Changes in gamma-aminobutyrate type A receptor subunit mRNAs, translation product expression, and receptor function during neuronal maturation in vitro.

Authors:  T M Zheng; W J Zhu; G Puia; S Vicini; D R Grayson; E Costa; H J Caruncho
Journal:  Proc Natl Acad Sci U S A       Date:  1994-11-08       Impact factor: 11.205

10.  Molecular and functional interaction between protocadherin-γC5 and GABAA receptors.

Authors:  Yanfang Li; Haiyan Xiao; Tzu-Ting Chiou; Hongbing Jin; Bevan Bonhomme; Celia P Miralles; Noelia Pinal; Rashid Ali; Weisheng V Chen; Tom Maniatis; Angel L De Blas
Journal:  J Neurosci       Date:  2012-08-22       Impact factor: 6.167
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