Literature DB >> 1376247

The acidic transcriptional activator GAL-VP16 acts on preformed template-committed complexes.

J White1, C Brou, J Wu, Y Lutz, V Moncollin, P Chambon.   

Abstract

The action of the chimeric acidic transcriptional activator GAL-VP16 has been investigated by performing a series of kinetic experiments using the detergent Sarkosyl as well as monoclonal antibodies which specifically inhibit GAL-VP16 DNA binding and transcriptional activation. GAL-VP16 binds to recognition site rapidly, remains bound after transcriptional initiation and is required to maintain stimulated levels of reinitiation. GAL-VP16 action, which appears to result in an increase in the number of preinitiation complexes formed, occurs after the formation of template-committed complexes composed of promoter-bound TFIIA (STF) and a partially purified TFIID fraction conferring GAL-VP16 responsiveness on a reconstituted basal transcription system. This TFIID fraction cannot be replaced by TFIIB or cloned TFIID. Our results suggest that GAL-VP16 activates step(s) in preinitiation complex assembly occurring after TFIID has bound.

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Year:  1992        PMID： 1376247      PMCID： PMC556690          DOI： 10.1002/j.1460-2075.1992.tb05282.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  51 in total

1.  Mechanism of action of a yeast activator: direct effect of GAL4 derivatives on mammalian TFIID-promoter interactions.

Authors:  M Horikoshi; M F Carey; H Kakidani; R G Roeder
Journal:  Cell       Date:  1988-08-26       Impact factor: 41.582

Review 2.  How eukaryotic transcriptional activators work.

Authors:  M Ptashne
Journal:  Nature       Date:  1988-10-20       Impact factor: 49.962

3.  A versatile in vivo and in vitro eukaryotic expression vector for protein engineering.

Authors:  S Green; I Issemann; E Sheer
Journal:  Nucleic Acids Res       Date:  1988-01-11       Impact factor: 16.971

4.  Cooperativity and hierarchical levels of functional organization in the SV40 enhancer.

Authors:  C Fromental; M Kanno; H Nomiyama; P Chambon
Journal:  Cell       Date:  1988-09-23       Impact factor: 41.582

5.  The human oestrogen receptor functions in yeast.

Authors:  D Metzger; J H White; P Chambon
Journal:  Nature       Date:  1988-07-07       Impact factor: 49.962

6.  A general transcription factor forms a stable complex with RNA polymerase B (II).

Authors:  X M Zheng; V Moncollin; J M Egly; P Chambon
Journal:  Cell       Date:  1987-07-31       Impact factor: 41.582

7.  Purification and characterization of a specific RNA polymerase II transcription factor.

Authors:  M Samuels; P A Sharp
Journal:  J Biol Chem       Date:  1986-02-15       Impact factor: 5.157

8.  Five intermediate complexes in transcription initiation by RNA polymerase II.

Authors:  S Buratowski; S Hahn; L Guarente; P A Sharp
Journal:  Cell       Date:  1989-02-24       Impact factor: 41.582

9.  Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.

Authors:  F W Studier; B A Moffatt
Journal:  J Mol Biol       Date:  1986-05-05       Impact factor: 5.469

10.  Factors involved in specific transcription by mammalian RNA polymerase II: purification, genetic specificity, and TATA box-promoter interactions of TFIID.

Authors:  N Nakajima; M Horikoshi; R G Roeder
Journal:  Mol Cell Biol       Date:  1988-10       Impact factor: 4.272
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  61 in total

1.  Herpes simplex virus type 1 ICP4 promotes transcription preinitiation complex formation by enhancing the binding of TFIID to DNA.

Authors:  B Grondin; N DeLuca
Journal:  J Virol       Date:  2000-12       Impact factor: 5.103

2.  Phosphorylation by p38MAPK and recruitment of SUG-1 are required for RA-induced RAR gamma degradation and transactivation.

Authors:  Maurizio Giannì; Annie Bauer; Enrico Garattini; Pierre Chambon; Cécile Rochette-Egly
Journal:  EMBO J       Date:  2002-07-15       Impact factor: 11.598

3.  Mechanism by which the IFN-beta enhanceosome activates transcription.

Authors:  J Yie; K Senger; D Thanos
Journal:  Proc Natl Acad Sci U S A       Date:  1999-11-09       Impact factor: 11.205

4.  Formation of an active tissue-specific chromatin domain initiated by epigenetic marking at the embryonic stem cell stage.

Authors:  Henrietta Szutorisz; Claudia Canzonetta; Andrew Georgiou; Cheok-Man Chow; László Tora; Niall Dillon
Journal:  Mol Cell Biol       Date:  2005-03       Impact factor: 4.272

5.  Sarkosyl block of transcription reinitiation by RNA polymerase II as visualized by the colliding polymerases reinitiation assay.

Authors:  M N Szentirmay; M Sawadogo
Journal:  Nucleic Acids Res       Date:  1994-12-11       Impact factor: 16.971

6.  A single GAL4 dimer can maximally activate transcription under physiological conditions.

Authors:  H E Xu; T Kodadek; S A Johnston
Journal:  Proc Natl Acad Sci U S A       Date:  1995-08-15       Impact factor: 11.205

7.  RNA polymerase II transcription complex assembly in nuclear extracts.

Authors:  C M Bral; J W Steinke; C J Kang; D O Peterson
Journal:  Gene Expr       Date:  1998

8.  Transcription activation by GC-boxes: evaluation of kinetic and equilibrium contributions.

Authors:  D Yean; J Gralla
Journal:  Nucleic Acids Res       Date:  1996-07-15       Impact factor: 16.971

9.  A cell-specific factor represses stimulation of transcription in vitro by transcriptional enhancer factor 1.

Authors:  S Chaudhary; C Brou; M E Valentin; N Burton; L Tora; P Chambon; I Davidson
Journal:  Mol Cell Biol       Date:  1994-08       Impact factor: 4.272

10.  Two distinct nuclear receptor interaction domains in NSD1, a novel SET protein that exhibits characteristics of both corepressors and coactivators.

Authors:  N Huang; E vom Baur; J M Garnier; T Lerouge; J L Vonesch; Y Lutz; P Chambon; R Losson
Journal:  EMBO J       Date:  1998-06-15       Impact factor: 11.598
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