Literature DB >> 1374074

Chemiluminescent universal probe for bacterial ribotyping.

C A Gustaferro1, D H Persing.   

Abstract

We describe a novel procedure for direct covalent coupling of horseradish peroxidase to rRNA and ribotyping by using enhanced chemiluminescence. Compared with their 32P-end-labeled counterparts, chemiluminescent rRNA probes are stable and easy to synthesize and provide results as good as or superior to those obtained with isotopic labeling. Direct chemiluminescent labeling of Escherichia coli rRNA produces a sensitive, universal probe suitable for clinical laboratory use in the investigation of nosocomial outbreaks.

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Year:  1992        PMID： 1374074      PMCID： PMC265214          DOI： 10.1128/jcm.30.4.1039-1041.1992

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  Nonradioactive nucleic acid detection by enhanced chemiluminescence using probes directly labeled with horseradish peroxidase.

Authors:  D Pollard-Knight; C A Read; M J Downes; L A Howard; M R Leadbetter; S A Pheby; E McNaughton; A Syms; M A Brady
Journal:  Anal Biochem       Date:  1990-02-15       Impact factor: 3.365

2.  A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA.

Authors:  T L Stull; J J LiPuma; T D Edlind
Journal:  J Infect Dis       Date:  1988-02       Impact factor: 5.226

3.  Ribosomal RNA gene restriction patterns provide increased sensitivity for typing Salmonella typhi strains.

Authors:  M Altwegg; F W Hickman-Brenner; J J Farmer
Journal:  J Infect Dis       Date:  1989-07       Impact factor: 5.226

4.  Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools.

Authors:  F Grimont; P A Grimont
Journal:  Ann Inst Pasteur Microbiol       Date:  1986 Sep-Oct

5.  Identification of Vibrio anguillarum in fish by using partial 16S rRNA sequences and a specific 16S rRNA oligonucleotide probe.

Authors:  A S Rehnstam; A Norqvist; H Wolf-Watz; A Hagström
Journal:  Appl Environ Microbiol       Date:  1989-08       Impact factor: 4.792

6.  DNA restriction digest and ribosomal RNA gene patterns of Campylobacter jejuni: a comparison with bio-, sero-, and bacteriophage-types of United Kingdom outbreak strains.

Authors:  R J Owen; J Hernandez; F Bolton
Journal:  Epidemiol Infect       Date:  1990-10       Impact factor: 2.451

7.  Identification of Francisella species and discrimination of type A and type B strains of F. tularensis by 16S rRNA analysis.

Authors:  M Forsman; G Sandström; B Jaurin
Journal:  Appl Environ Microbiol       Date:  1990-04       Impact factor: 4.792

8.  DNA polymorphisms among Escherichia coli isolated from bacteriuric women.

Authors:  J J LiPuma; T L Stull; S E Dasen; K A Pidcock; D Kaye; O M Korzeniowski
Journal:  J Infect Dis       Date:  1989-03       Impact factor: 5.226

9.  Use of an rRNA probe and restriction endonuclease analysis to fingerprint Pasteurella multocida isolated from turkeys and wildlife.

Authors:  K P Snipes; D C Hirsh; R W Kasten; L M Hansen; D W Hird; T E Carpenter; R H McCapes
Journal:  J Clin Microbiol       Date:  1989-08       Impact factor: 5.948

10.  rRNA gene restriction patterns of Haemophilus influenzae biogroup aegyptius strains associated with Brazilian purpuric fever.

Authors:  K Irino; F Grimont; I Casin; P A Grimont
Journal:  J Clin Microbiol       Date:  1988-08       Impact factor: 5.948
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  7 in total

Review 1.  Molecular biology and infections of the gut.

Authors:  N P Mapstone; P Quirke
Journal:  Gut       Date:  1992-11       Impact factor: 23.059

Review 2.  Use of ribotyping in epidemiological surveillance of nosocomial outbreaks.

Authors:  E H Bingen; E Denamur; J Elion
Journal:  Clin Microbiol Rev       Date:  1994-07       Impact factor: 26.132

3.  Assessment of a chemiluminescent universal probe for taxonomical and epidemiological investigations of Aeromonas sp isolates.

Authors:  P E Carey; A Eley; M H Wilcox
Journal:  J Clin Pathol       Date:  1994-07       Impact factor: 3.411

4.  PCR diagnosis of typhoid fever.

Authors:  J H Song
Journal:  J Clin Microbiol       Date:  1994-08       Impact factor: 5.948

5.  Random amplified polymorphic DNA assay is less discriminant than pulsed-field gel electrophoresis for typing strains of methicillin-resistant Staphylococcus aureus.

Authors:  P Saulnier; C Bourneix; G Prévost; A Andremont
Journal:  J Clin Microbiol       Date:  1993-04       Impact factor: 5.948

6.  Typing of Candida krusei clinical isolates by restriction endonuclease analysis and hybridization with CkF1,2 DNA probe.

Authors:  A Carlotti; R Grillot; A Couble; J Villard
Journal:  J Clin Microbiol       Date:  1994-07       Impact factor: 5.948

7.  Genetic stability of Borrelia burgdorferi recovered from chronically infected immunocompetent mice.

Authors:  D H Persing; D Mathiesen; D Podzorski; S W Barthold
Journal:  Infect Immun       Date:  1994-08       Impact factor: 3.441
  7 in total

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