Literature DB >> 1374

Purification and properties of polyol dehydrogenase from Cephalosporium chrysogenus.

S Birken, M A Pisano.   

Abstract

A polyol dehydrogenase of broad specificity was purified 178-fold from extracts of the filamentous fungus Cephalosporium chrysogenum. The enzyme was found to act as an oxido-reductase in two substrate-coenzyme systems: D-sorbitol (or xylitol)-nicotinamide-adenine dinucleotide (NAD) and D-mannitol-nicotinamide adenine dinucleotide phosphate (NADP). The dehydrogenase was composed of five isozymes, which, as a mixture, exhibited these properties: Km to D-sorbitol and D-mannitol, 7.15 to 10(-2) M; PH optimum, 9 to 10; molecular weight, 300,000 subunit weight, 29,000; PI, 5.8 to 7.5. The NADP-linked activity was labile to treatment with heat or ethylenediaminetetraacetic acid. Mixed substrate assays support the hypothesis that both NAD-, and NADP-linked activities are associated with isozymes of a single dehydrogenase.

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Year:  1976        PMID： 1374      PMCID： PMC233356          DOI： 10.1128/jb.125.1.225-232.1976

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  13 in total

1.  HEXITOL DEHYDROGENASES OF BACILLUS SUBTILIS.

Authors:  S B HORWITZ; N O KAPLAN
Journal:  J Biol Chem       Date:  1964-03       Impact factor: 5.157

2.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

3.  Metabolism of d-xylose by moulds.

Authors:  C CHIANG; S G KNIGHT
Journal:  Nature       Date:  1960-10-01       Impact factor: 49.962

4.  [The mechanism of the formation of seminal fructose and fetal fructose].

Authors:  H G HERS
Journal:  Biochim Biophys Acta       Date:  1960-01-01

5.  MULTIPLE FORMS OF ENZYMES: TISSUE, ONTOGENETIC, AND SPECIES SPECIFIC PATTERNS.

Authors:  C L Markert; F Møller
Journal:  Proc Natl Acad Sci U S A       Date:  1959-05       Impact factor: 11.205

6.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

7.  Studies on polyol metabolism in Aspergillus niger. 3. Purification and properties of sorbitol dehydrogenase.

Authors:  B M Desai; V V Modi; V K Shah
Journal:  Arch Mikrobiol       Date:  1969

Review 8.  Isoelectric focusing in pH gradients--a technique for fractionation and characterization of ampholytes.

Authors:  H Haglund
Journal:  Methods Biochem Anal       Date:  1971

9.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

10.  Pentose metabolism in Candida. II. The diphosphopyridine nucleotide-specific polyol dehydrogenase of Candida utilis.

Authors:  M CHAKRAVORTY; L A VEIGA; M BACILA; B L HORECKER
Journal:  J Biol Chem       Date:  1962-04       Impact factor: 5.157
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  6 in total

1.  Purification and characterization of a novel erythrose reductase from Candida magnoliae.

Authors:  Jung-Kul Lee; Sang-Yong Kim; Yeon-Woo Ryu; Jin-Ho Seo; Jung-Hoe Kim
Journal:  Appl Environ Microbiol       Date:  2003-07       Impact factor: 4.792

2.  Fumarate-mediated inhibition of erythrose reductase, a key enzyme for erythritol production by Torula corallina.

Authors:  Jung-Kul Lee; Bong-Seong Koo; Sang-Yong Kim
Journal:  Appl Environ Microbiol       Date:  2002-09       Impact factor: 4.792

3.  Characterization of the Escherichia coli Antifungal Protein PPEBL21.

Authors:  V Yadav; R Mandhan; M Kumar; J Gupta; G L Sharma
Journal:  Int J Microbiol       Date:  2010-05-17

4.  Cloning, purification and characterization of an NAD-Dependent D-Arabitol dehydrogenase from acetic acid bacterium, Acetobacter suboxydans.

Authors:  Hairong Cheng; Zilong Li; Ning Jiang; Zixin Deng
Journal:  Protein J       Date:  2009-08       Impact factor: 2.371

5.  1,8-dihydroxynaphthalene (DHN)-melanin biosynthesis inhibitors increase erythritol production in Torula corallina, and DHN-melanin inhibits erythrose reductase.

Authors:  Jung-Kul Lee; Hyung-Moo Jung; Sang-Yong Kim
Journal:  Appl Environ Microbiol       Date:  2003-06       Impact factor: 4.792

6.  Purification and characterization of a novel mannitol dehydrogenase from a newly isolated strain of Candida magnoliae.

Authors:  Jung-Kul Lee; Bong-Seong Koo; Sang-Yong Kim; Hyung-Hwan Hyun
Journal:  Appl Environ Microbiol       Date:  2003-08       Impact factor: 4.792
  6 in total

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