OBJECTIVE: Substance P and somatostatin are neuropeptides found in peripheral sensory nerves. In vitro, these have opposing effects on inflammatory cells. We compared the effects of these peptides on the activation of neutrophils. METHODS: Neutrophils were isolated from healthy volunteers, and chemotaxis, superoxide anion generation, aggregation, and changes in cytosolic calcium and GTPase activity were measured in the presence of substance P, somatostatin, and the chemoattractant FMLP. RESULTS: Substance P was an effective chemoattractant, 20% as potent as FMLP at equimolar concentrations. Substance P also stimulated GTPase activity in neutrophil plasma membranes. Somatostatin did not activate neutrophils; however, it effectively inhibited neutrophil chemotaxis and GTPase activity provoked by substance P, but not by FMLP. CONCLUSIONS: These studies demonstrate that substance P can effectively stimulate chemotaxis, possibly via effects on a GTP-binding protein distinct from that triggered by FMLP, and that somatostatin is a selective antagonist of substance P. The biochemical specificities of these peptides on cells may modulate neurogenic inflammation at the local level.
OBJECTIVE:Substance P and somatostatin are neuropeptides found in peripheral sensory nerves. In vitro, these have opposing effects on inflammatory cells. We compared the effects of these peptides on the activation of neutrophils. METHODS: Neutrophils were isolated from healthy volunteers, and chemotaxis, superoxide anion generation, aggregation, and changes in cytosolic calcium and GTPase activity were measured in the presence of substance P, somatostatin, and the chemoattractant FMLP. RESULTS:Substance P was an effective chemoattractant, 20% as potent as FMLP at equimolar concentrations. Substance P also stimulated GTPase activity in neutrophil plasma membranes. Somatostatin did not activate neutrophils; however, it effectively inhibited neutrophil chemotaxis and GTPase activity provoked by substance P, but not by FMLP. CONCLUSIONS: These studies demonstrate that substance P can effectively stimulate chemotaxis, possibly via effects on a GTP-binding protein distinct from that triggered by FMLP, and that somatostatin is a selective antagonist of substance P. The biochemical specificities of these peptides on cells may modulate neurogenic inflammation at the local level.
Authors: Zsuzsanna Helyes; Erika Pintér; Katalin Sándor; Krisztián Elekes; Agnes Bánvölgyi; Dániel Keszthelyi; Eva Szoke; Dániel M Tóth; Zoltán Sándor; László Kereskai; Gábor Pozsgai; Jeremy P Allen; Piers C Emson; Adrienn Markovics; János Szolcsányi Journal: Proc Natl Acad Sci U S A Date: 2009-07-21 Impact factor: 11.205
Authors: C J Wiedermann; N Reinisch; M Niedermühlbichler; H Braunsteiner Journal: Naunyn Schmiedebergs Arch Pharmacol Date: 1993-03 Impact factor: 3.000
Authors: L J Crofford; H Sano; K Karalis; E L Webster; E A Goldmuntz; G P Chrousos; R L Wilder Journal: J Clin Invest Date: 1992-12 Impact factor: 14.808
Authors: Zoltán Varecza; Krisztián Elekes; Terézia László; Anikó Perkecz; Erika Pintér; Zoltán Sándor; János Szolcsányi; Dániel Keszthelyi; Arpád Szabó; Katalin Sándor; Tamás F Molnár; Zalán Szántó; Judit E Pongrácz; Zsuzsanna Helyes Journal: J Histochem Cytochem Date: 2009-08-17 Impact factor: 2.479