Literature DB >> 1373206

Reverse transcriptase of human immunodeficiency virus type 1: functionality of subunits of the heterodimer in DNA synthesis.

Z Hostomsky1, Z Hostomska, T B Fu, J Taylor.   

Abstract

From an in vitro analysis of the DNA-synthesizing abilities of certain specifically mutated forms of the heterodimeric reverse transcriptase of human immunodeficiency virus type 1, we can conclude that in a heterodimer, the functionality of p66 is necessary while the functionality of the p51 subunit is not needed. Conversely, p51 is not able to catalyze DNA synthesis when associated with p66, and yet when the p66 protein is absent, p51 can function. These conclusions applied to DNA synthesis on heteropolymeric RNA and DNA templates.

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Year:  1992        PMID： 1373206      PMCID： PMC241083     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  16 in total

1.  Dimerization of human immunodeficiency virus type 1 reverse transcriptase. A target for chemotherapeutic intervention.

Authors:  T Restle; B Müller; R S Goody
Journal:  J Biol Chem       Date:  1990-06-05       Impact factor: 5.157

2.  Characterization of highly immunogenic p66/p51 as the reverse transcriptase of HTLV-III/LAV.

Authors:  F di Marzo Veronese; T D Copeland; A L DeVico; R Rahman; S Oroszlan; R C Gallo; M G Sarngadharan
Journal:  Science       Date:  1986-03-14       Impact factor: 47.728

3.  Murine leukemia virus pol gene products: analysis with antisera generated against reverse transcriptase and endonuclease fusion proteins expressed in Escherichia coli.

Authors:  S C Hu; D L Court; M Zweig; J G Levin
Journal:  J Virol       Date:  1986-10       Impact factor: 5.103

4.  Co-expression of the subunits of the heterodimer of HIV-1 reverse transcriptase in Escherichia coli.

Authors:  B Müller; T Restle; S Weiss; M Gautel; G Sczakiel; R S Goody
Journal:  J Biol Chem       Date:  1989-08-25       Impact factor: 5.157

5.  Site-directed mutagenesis of the conserved Asp-443 and Asp-498 carboxy-terminal residues of HIV-1 reverse transcriptase.

Authors:  V Mizrahi; M T Usdin; A Harington; L R Dudding
Journal:  Nucleic Acids Res       Date:  1990-09-25       Impact factor: 16.971

6.  Proteolytic release and crystallization of the RNase H domain of human immunodeficiency virus type 1 reverse transcriptase.

Authors:  Z Hostomska; D A Matthews; J F Davies; B R Nodes; Z Hostomsky
Journal:  J Biol Chem       Date:  1991-08-05       Impact factor: 5.157

7.  A novel dipyridodiazepinone inhibitor of HIV-1 reverse transcriptase acts through a nonsubstrate binding site.

Authors:  J C Wu; T C Warren; J Adams; J Proudfoot; J Skiles; P Raghavan; C Perry; I Potocki; P R Farina; P M Grob
Journal:  Biochemistry       Date:  1991-02-26       Impact factor: 3.162

8.  High-level expression of self-processed HIV-1 protease in Escherichia coli using a synthetic gene.

Authors:  Z Hostomsky; K Appelt; R C Ogden
Journal:  Biochem Biophys Res Commun       Date:  1989-06-30       Impact factor: 3.575

9.  Structural characterization of reverse transcriptase and endonuclease polypeptides of the acquired immunodeficiency syndrome retrovirus.

Authors:  M M Lightfoote; J E Coligan; T M Folks; A S Fauci; M A Martin; S Venkatesan
Journal:  J Virol       Date:  1986-11       Impact factor: 5.103

10.  Site-specific mutagenesis of AIDS virus reverse transcriptase.

Authors:  B A Larder; D J Purifoy; K L Powell; G Darby
Journal:  Nature       Date:  1987 Jun 25-Jul 1       Impact factor: 49.962
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  30 in total

1.  Insertion of a peptide from MuLV RT into the connection subdomain of HIV-1 RT results in a functionally active chimeric enzyme in monomeric conformation.

Authors:  P K Pandey; N Kaushik; T T Talele; P N Yadav; V N Pandey
Journal:  Mol Cell Biochem       Date:  2001-09       Impact factor: 3.396

2.  Molecular matchmaking: NNRTIs can enhance the dimerization of HIV type 1 reverse transcriptase.

Authors:  S H Hughes
Journal:  Proc Natl Acad Sci U S A       Date:  2001-06-19       Impact factor: 11.205

3.  The nature of the N-terminal amino acid residue of HIV-1 RNase H is critical for the stability of reverse transcriptase in viral particles.

Authors:  Guney Boso; Claes Örvell; Nikunj V Somia
Journal:  J Virol       Date:  2014-11-12       Impact factor: 5.103

4.  When retroviral reverse transcriptases reach the end of their RNA templates.

Authors:  T B Fu; J Taylor
Journal:  J Virol       Date:  1992-07       Impact factor: 5.103

5.  Asymmetric subunit organization of heterodimeric Rous sarcoma virus reverse transcriptase alphabeta: localization of the polymerase and RNase H active sites in the alpha subunit.

Authors:  S Werner; B M Wöhrl
Journal:  J Virol       Date:  2000-04       Impact factor: 5.103

6.  HIV-1 protease dimer interface mutations that compensate for viral reverse transcriptase instability in infectious virions.

Authors:  Isabel Olivares; Alok Mulky; Peter I Boross; József Tözsér; John C Kappes; Cecilio López-Galíndez; Luis Menéndez-Arias
Journal:  J Mol Biol       Date:  2007-07-03       Impact factor: 5.469

7.  Structural basis of asymmetry in the human immunodeficiency virus type 1 reverse transcriptase heterodimer.

Authors:  J Wang; S J Smerdon; J Jäger; L A Kohlstaedt; P A Rice; J M Friedman; T A Steitz
Journal:  Proc Natl Acad Sci U S A       Date:  1994-07-19       Impact factor: 11.205

8.  Reverse transcription in hepatitis B viruses is primed by a tyrosine residue of the polymerase.

Authors:  F Zoulim; C Seeger
Journal:  J Virol       Date:  1994-01       Impact factor: 5.103

9.  Reverse transcriptase and protease activities of avian leukosis virus Gag-Pol fusion proteins expressed in insect cells.

Authors:  L Stewart; V M Vogt
Journal:  J Virol       Date:  1993-12       Impact factor: 5.103

10.  Crystal structure of human immunodeficiency virus type 1 reverse transcriptase complexed with double-stranded DNA at 3.0 A resolution shows bent DNA.

Authors:  A Jacobo-Molina; J Ding; R G Nanni; A D Clark; X Lu; C Tantillo; R L Williams; G Kamer; A L Ferris; P Clark
Journal:  Proc Natl Acad Sci U S A       Date:  1993-07-01       Impact factor: 11.205
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