Literature DB >> 1372272

RNase H activity of HIV reverse transcriptases is confined exclusively to the dimeric forms.

T Restle1, B Müller, R S Goody.   

Abstract

A method for the rapid preparation of a defined substrate to monitor RNase H activity has been developed. Using this substrate, we have investigated the RNase H activities of the different forms of recombinant HIV-1 and HIV-2 reverse transcriptase (RT) in detail. As we report here, RNase H activity is associated only with the dimeric forms (p51/p66 or p66/p66) of the enzymes.

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Year:  1992        PMID： 1372272     DOI： 10.1016/0014-5793(92)80172-d

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  20 in total

1.  Structure-activity relationships in HIV-1 reverse transcriptase revealed by radiation target analysis.

Authors:  Nicolas Sluis-Cremer; Ellis Kempner; Michael A Parniak
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Review 2.  Conformational changes in HIV-1 reverse transcriptase induced by nonnucleoside reverse transcriptase inhibitor binding.

Authors:  Nicolas Sluis-Cremer; N Alpay Temiz; Ivet Bahar
Journal:  Curr HIV Res       Date:  2004-10       Impact factor: 1.581

3.  The cellular antiviral protein APOBEC3G interacts with HIV-1 reverse transcriptase and inhibits its function during viral replication.

Authors:  Xiaoxia Wang; Zhujun Ao; Liyu Chen; Gary Kobinger; Jinyu Peng; Xiaojian Yao
Journal:  J Virol       Date:  2012-02-01       Impact factor: 5.103

4.  Asymmetric subunit organization of heterodimeric Rous sarcoma virus reverse transcriptase alphabeta: localization of the polymerase and RNase H active sites in the alpha subunit.

Authors:  S Werner; B M Wöhrl
Journal:  J Virol       Date:  2000-04       Impact factor: 5.103

5.  Fusion with an RNA binding domain to confer target RNA specificity to an RNase: design and engineering of Tat-RNase H that specifically recognizes and cleaves HIV-1 RNA in vitro.

Authors:  Y F Melekhovets; S Joshi
Journal:  Nucleic Acids Res       Date:  1996-05-15       Impact factor: 16.971

6.  Purification and characterization of an active human immunodeficiency virus type 1 RNase H domain.

Authors:  J S Smith; M J Roth
Journal:  J Virol       Date:  1993-07       Impact factor: 5.103

7.  Hydroxyl radical footprint analysis of human immunodeficiency virus reverse transcriptase-template.primer complexes.

Authors:  W Metzger; T Hermann; O Schatz; S F Le Grice; H Heumann
Journal:  Proc Natl Acad Sci U S A       Date:  1993-07-01       Impact factor: 11.205

8.  Multiparameter single-molecule fluorescence spectroscopy reveals heterogeneity of HIV-1 reverse transcriptase:primer/template complexes.

Authors:  P J Rothwell; S Berger; O Kensch; S Felekyan; M Antonik; B M Wöhrl; T Restle; R S Goody; C A M Seidel
Journal:  Proc Natl Acad Sci U S A       Date:  2003-02-10       Impact factor: 11.205

9.  Selective killing of human immunodeficiency virus infected cells by non-nucleoside reverse transcriptase inhibitor-induced activation of HIV protease.

Authors:  Dirk Jochmans; Maria Anders; Inge Keuleers; Liesbeth Smeulders; Hans-Georg Kräusslich; Günter Kraus; Barbara Müller
Journal:  Retrovirology       Date:  2010-10-15       Impact factor: 4.602

10.  Kinetics of association and dissociation of HIV-1 reverse transcriptase subunits.

Authors:  Carl F Venezia; Brendan J Meany; Valerie A Braz; Mary D Barkley
Journal:  Biochemistry       Date:  2009-09-29       Impact factor: 3.162
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