Literature DB >> 1371921

Inhibition of IgE-mediated histamine release by myosin light chain kinase inhibitors.

S Kitani1, R Teshima, Y Morita, K Ito, Y Matsuda, Y Nonomura.   

Abstract

Wortmannin, a specific inhibitor of myosin light chain kinase (MLCK) blocked IgE mediated histamine release from rat basophilic leukemia cell (RBL-2H3) and human basophils dose-dependently. Its IC50 was 20 nM for RBL-2H3 cells and 30 nM for human basophils. There was complete inhibition at the concentration of 1 microM. Wortmannin inhibited partially the A23187 induced histamine release from RBL-2H3 cells (40% inhibition at 1 microM). This inhibition was not accompanied by any significant effect on cytosolic free calcium concentration [( Ca2+]i). KT5926, another MLCK inhibitor, inhibited histamine release comparably with wortmannin and blocked to some degree the increase of [Ca2+]i in RBL-2H3 cells. Thus, the phosphorylation of myosin seems to be involved in signal transduction through Fc epsilon RI.

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Year:  1992        PMID： 1371921     DOI： 10.1016/0006-291x(92)91607-r

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  6 in total

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Authors:  H Sasaki; M Nakamura; T Ohno; Y Matsuda; Y Yuda; Y Nonomura
Journal:  Proc Natl Acad Sci U S A       Date:  1995-03-14       Impact factor: 11.205

5.  Wortmannin blocks lipid and protein kinase activities associated with PI 3-kinase and inhibits a subset of responses induced by Fc epsilon R1 cross-linking.

Authors:  S A Barker; K K Caldwell; A Hall; A M Martinez; J R Pfeiffer; J M Oliver; B S Wilson
Journal:  Mol Biol Cell       Date:  1995-09       Impact factor: 4.138

6.  Lipid Cubic Systems for Sustained and Controlled Delivery of Antihistamine Drugs.

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  6 in total

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