Literature DB >> 1371919

Purification of nitric oxide synthase from bovine brain: immunological characterization and tissue distribution.

H Ohshima1, S Oguchi, H Adachi, S Iida, H Suzuki, T Sugimura, H Esumi.   

Abstract

Nitric oxide (NO) synthase (EC 1.14.23) was purified to homogeneity from bovine cerebrum. The molecular weight of NO synthase was estimated to be 150 kDa by both SDS/PAGE and gel filtration at high salt concentration. For activity, the enzyme required NADPH, Ca2+, calmodulin and tetrahydrobiopterin as cofactors. Rabbit polyclonal antibody to bovine brain NO synthase reacted with 150 kDa NO synthase in various bovine and rat organs, including the brain, pituitary and adrenal glands, but not with that in stimulated macrophages, indicating that there are at least two immunologically distinct NO synthases.

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Year:  1992        PMID： 1371919     DOI： 10.1016/0006-291x(92)91634-3

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  7 in total

1.  Pathological characterization of astrocytic hyaline inclusions in familial amyotrophic lateral sclerosis.

Authors:  S Kato; H Hayashi; K Nakashima; E Nanba; M Kato; A Hirano; I Nakano; K Asayama; E Ohama
Journal:  Am J Pathol       Date:  1997-08       Impact factor: 4.307

2.  Nitric oxide synthase in ferret brain: localization and characterization.

Authors:  T Matsumoto; J A Mitchell; H H Schmidt; K L Kohlhaas; T D Warner; U Förstermann; F Murad
Journal:  Br J Pharmacol       Date:  1992-11       Impact factor: 8.739

3.  [Determination of NO-synthase activity in the brain (new method)].

Authors:  A I Tsapin; M Iu Stepanichev; M L Libe; N V Guliaeva
Journal:  Biull Eksp Biol Med       Date:  1994-01

Review 4.  Nitric oxide synthases in mammals.

Authors:  R G Knowles; S Moncada
Journal:  Biochem J       Date:  1994-03-01       Impact factor: 3.857

5.  Purification and characterization of nitric oxide synthase (NOSNoc) from a Nocardia species.

Authors:  Y Chen; J P Rosazza
Journal:  J Bacteriol       Date:  1995-09       Impact factor: 3.490

6.  Endothelial nitric oxide synthase: molecular cloning and characterization of a distinct constitutive enzyme isoform.

Authors:  S Lamas; P A Marsden; G K Li; P Tempst; T Michel
Journal:  Proc Natl Acad Sci U S A       Date:  1992-07-15       Impact factor: 11.205

Review 7.  Nitric oxide synthase isozymes antibodies.

Authors:  J S Pollock; U Förstermann; W R Tracey; M Nakane
Journal:  Histochem J       Date:  1995-10
  7 in total

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