Literature DB >> 1371476

Imidazole-SDS-Zn reverse staining of proteins in gels containing or not SDS and microsequence of individual unmodified electroblotted proteins.

M L Ortiz1, M Calero, C Fernandez Patron, C F Patron, L Castellanos, E Mendez.   

Abstract

A reverse staining procedure is described for the detection of proteins in acrylamide and agarose gels with and without SDS. Protein detection occurs a few minutes after electrophoresis. The sensitivity on acrylamide gels is higher than that of Coomassie blue staining either on acrylamide gels or on electrotransferred membranes. Sequencing of protein bands only detected by reverse staining on the gel and not by Coomassie blue is demonstrated.

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Year:  1992        PMID： 1371476     DOI： 10.1016/0014-5793(92)80309-5

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  10 in total

1.  Processing of anti-mullerian hormone regulates receptor activation by a mechanism distinct from TGF-beta.

Authors:  Nathalie di Clemente; Soazik P Jamin; Alexey Lugovskoy; Paul Carmillo; Christian Ehrenfels; Jean-Yves Picard; Adrian Whitty; Nathalie Josso; R Blake Pepinsky; Richard L Cate
Journal:  Mol Endocrinol       Date:  2010-09-22

2.  Quantitative proteomics: assessing the spectrum of in-gel protein detection methods.

Authors:  Victoria J Gauci; Elise P Wright; Jens R Coorssen
Journal:  J Chem Biol       Date:  2010-06-19

3.  Evidence for the possible involvement of calmodulin in regulation of steady state levels of Hsp90 family members (Hsp87 and Hsp85) in response to heat shock in sorghum.

Authors:  Amardeep Singh Virdi; Ashwani Pareek; Prabhjeet Singh
Journal:  Plant Signal Behav       Date:  2011-03-01

4.  Direct mass-spectrometric identification of Arg296 and Arg299 as the methylation sites of hnRNP K protein for methyltransferase PRMT1.

Authors:  Yi-Ying Chiou; Wey-Jinq Lin; Shu-Ling Fu; Chao-Hsiung Lin
Journal:  Protein J       Date:  2007-02       Impact factor: 2.371

5.  Phosphorylation of rat liver heterogeneous nuclear ribonucleoproteins A2 and C can be modulated by calmodulin.

Authors:  R Bosser; M Faura; J Serratosa; J Renau-Piqueras; M Pruschy; O Bachs
Journal:  Mol Cell Biol       Date:  1995-02       Impact factor: 4.272

6.  Fibroin-modulator-binding protein-1 (FMBP-1) contains a novel DNA-binding domain, repeats of the score and three amino acid peptide (STP), conserved from Caenorhabditis elegans to humans.

Authors:  Shigeharu Takiya; Tetsurou Ishikawa; Katsuya Ohtsuka; Yoshinori Nishita; Yoshiaki Suzuki
Journal:  Nucleic Acids Res       Date:  2005-02-01       Impact factor: 16.971

7.  The intrinsically disordered distal face of nucleoplasmin recognizes distinct oligomerization states of histones.

Authors:  Isbaal Ramos; Noelia Fernández-Rivero; Rocío Arranz; Kerman Aloria; Ron Finn; Jesús M Arizmendi; Juan Ausió; José María Valpuesta; Arturo Muga; Adelina Prado
Journal:  Nucleic Acids Res       Date:  2013-10-10       Impact factor: 16.971

8.  Nucleolar proteins Bfr2 and Enp2 interact with DEAD-box RNA helicase Dbp4 in two different complexes.

Authors:  Sahar Soltanieh; Martin Lapensée; François Dragon
Journal:  Nucleic Acids Res       Date:  2013-12-18       Impact factor: 16.971

9.  In vitro assembly of a functional human CDK7-cyclin H complex requires MAT1, a novel 36 kDa RING finger protein.

Authors:  J P Tassan; M Jaquenoud; A M Fry; S Frutiger; G J Hughes; E A Nigg
Journal:  EMBO J       Date:  1995-11-15       Impact factor: 11.598

10.  Establishment of a bacterial expression system and immunoassay platform for the major capsid protein of HcRNAV, a dinoflagellate-infecting RNA virus.

Authors:  Kei Wada; Kei Kimura; Akifumi Hasegawa; Keiichi Fukuyama; Keizo Nagasaki
Journal:  Microbes Environ       Date:  2012-10-05       Impact factor: 2.912
  10 in total

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