Literature DB >> 1370979

Overproduction of the RecD polypeptide sensitizes Escherichia coli cells to gamma-radiation.

K Brcić-Kostić1, I Stojiljković, E Salaj-Smic, Z Trgovcević.   

Abstract

We investigated DNA metabolism in Escherichia coli cells carrying the multicopy recD+ plasmid (pKI13). In the presence of pKI13, the cellular level of the recD gene product (RecD polypeptide) is amplified at least 60-fold. Overproduction of the RecD polypeptide has no effect on UV repair and conjugational recombination. In contrast, high cellular levels of this polypeptide sensitize wild-type cells to gamma-radiation; also, they increase the rate of radiation-induced DNA degradation. A possible mechanism for the enhancement of gamma-ray-induced killing by large amounts of the RecD polypeptide is discussed.

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Year:  1992        PMID： 1370979     DOI： 10.1016/0165-7992(92)90046-k

Source DB:  PubMed          Journal:  Mutat Res        ISSN： 0027-5107            Impact factor:   2.433


  5 in total

Review 1.  RecBCD enzyme and the repair of double-stranded DNA breaks.

Authors:  Mark S Dillingham; Stephen C Kowalczykowski
Journal:  Microbiol Mol Biol Rev       Date:  2008-12       Impact factor: 11.056

Review 2.  Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda.

Authors:  A Kuzminov
Journal:  Microbiol Mol Biol Rev       Date:  1999-12       Impact factor: 11.056

Review 3.  Biochemistry of homologous recombination in Escherichia coli.

Authors:  S C Kowalczykowski; D A Dixon; A K Eggleston; S D Lauder; W M Rehrauer
Journal:  Microbiol Rev       Date:  1994-09

4.  Chromosomal lesion suppression and removal in Escherichia coli via linear DNA degradation.

Authors:  Anabel Miranda; Andrei Kuzminov
Journal:  Genetics       Date:  2003-04       Impact factor: 4.562

5.  Interaction with the recombination hot spot chi in vivo converts the RecBCD enzyme of Escherichia coli into a chi-independent recombinase by inactivation of the RecD subunit.

Authors:  A Köppen; S Krobitsch; B Thoms; W Wackernagel
Journal:  Proc Natl Acad Sci U S A       Date:  1995-07-03       Impact factor: 11.205
  5 in total

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