Literature DB >> 1370930

HBsAg in urine: a new approach for the detection of urinary antigens.

S Badur1, L Grangeot-Keros, J Pillot.   

Abstract

In order to define the optimal conditions for detection of microbial antigens in urine, urinary HBsAg excreted during hepatitis B was chosen as a model. Using commercial kits, which mainly involve anti-discontinuous epitopes, we found urinary HBsAg in only 50% of patients with HBsAg in their sera. In contrast, with an inhibition method involving a monoclonal antibody recognizing a continuous epitope, urinary HBsAg was found in 100% of these patients. Structural analysis of HBsAg showed that urinary HBsAg is denatured; it can escape detection by commercial kits well fitted for detection of native serum HBsAg. General implications for the revelation of urinary microbial antigens are discussed.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1370930      PMCID： PMC1554269          DOI： 10.1111/j.1365-2249.1992.tb02991.x

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN： 0009-9104            Impact factor:   4.330


  11 in total

1.  Role of saliva, urine and feces in the transmission of type B hepatitis.

Authors:  V M Villarejos; K A Visoná; A Gutiérrez; A Rodríguez
Journal:  N Engl J Med       Date:  1974-12-26       Impact factor: 91.245

2.  Hepatitis-B antigen in saliva, urine and tears.

Authors:  S B Vittal; D Dourdourekas; F Steigmann
Journal:  Am J Gastroenterol       Date:  1974-02       Impact factor: 10.864

3.  Analysis of the vaccinating group specificity a of HBsAg with monoclonal antibodies. Identification of continuous and discontinuous epitopes.

Authors:  J Pillot; M M Riottot; C Geneste; L Phalente; R Mangalo
Journal:  Dev Biol Stand       Date:  1984

4.  Failure of tests to detect pneumococcal antigens in urine.

Authors:  L Lebrun; M Guibert; J Pillot
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1988-02       Impact factor: 3.267

5.  Hepatitis B surface antigen in urine of renal transplant recipients.

Authors:  L Kaiser; T J Kelly; M J Patterson; T V Sanchez; R S Shapiro; G H Mayor
Journal:  Ann Intern Med       Date:  1981-06       Impact factor: 25.391

6.  Physical and immunologic properties of pneumococcal capsular polysaccharide produced during human infection.

Authors:  J D Coonrod
Journal:  J Immunol       Date:  1974-06       Impact factor: 5.422

7.  Hepatitis B virus contains pre-S gene-encoded domains.

Authors:  A R Neurath; S B Kent; N Strick; P Taylor; C E Stevens
Journal:  Nature       Date:  1985 May 9-15       Impact factor: 49.962

8.  Immunochemical structure of the hepatitis B surface antigen vaccine--I. Treatment of immobilized HBsAg by dissociation agents with or without enzymatic digestion and identification of polypeptides by protein blotting.

Authors:  J Pillot; M A Petit
Journal:  Mol Immunol       Date:  1984-01       Impact factor: 4.407

9.  Immunochemical structure of the hepatitis B surface antigen vaccine--II. Analysis of antibody responses in human sera against the envelope proteins.

Authors:  M A Petit; P Maillard; F Capel; J Pillot
Journal:  Mol Immunol       Date:  1986-05       Impact factor: 4.407

10.  Immunization of mice and rabbits by intrasplenic deposition of nanogram quantities of protein attached to Sepharose beads or nitrocellulose paper strips.

Authors:  B O Nilsson; P C Svalander; A Larsson
Journal:  J Immunol Methods       Date:  1987-05-04       Impact factor: 2.303
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.