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Literature DB >> 1369475

DnaK-mediated alterations in human growth hormone protein inclusion bodies.

Abstract

Protein overproduction in microbes frequently results in protein misfolding and aggregation though the molecular basis for this process is unclear. The HSP70 chaperonin, DnaK, was identified as an important factor controlling heterologous protein aggregation in Escherichia coli. Co-overproduction of DnaK significantly reduced human growth hormone (HGH) protein inclusion body formation and the extent of HGH aggregation.

Entities: Disease Gene Species

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Year: 1992 PMID： 1369475 DOI： 10.1038/nbt0392-301

Source DB: PubMed Journal: Biotechnology (N Y) ISSN： 0733-222X

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Cited

13 in total

1. Monoclonal antibody recognition and function of a DnaK (HSP70) epitope found in gram-negative bacteria.

Authors: J Krska; T Elthon; P Blum
Journal: J Bacteriol Date: 1993-10 Impact factor: 3.490

2. Combined effects of the signal sequence and the major chaperone proteins on the export of human cytokines in Escherichia coli.

Authors: H Bergès; E Joseph-Liauzun; O Fayet
Journal: Appl Environ Microbiol Date: 1996-01 Impact factor: 4.792

3. Heat shock proteins DnaJ, DnaK, and GrpE stimulate P1 plasmid replication by promoting initiator binding to the origin.

Authors: S Sozhamannan; D K Chattoraj
Journal: J Bacteriol Date: 1993-06 Impact factor: 3.490

DnaK-mediated alterations in human growth hormone protein inclusion bodies.

1. Monoclonal antibody recognition and function of a DnaK (HSP70) epitope found in gram-negative bacteria.

2. Combined effects of the signal sequence and the major chaperone proteins on the export of human cytokines in Escherichia coli.

3. Heat shock proteins DnaJ, DnaK, and GrpE stimulate P1 plasmid replication by promoting initiator binding to the origin.

4. VapC6, a ribonucleolytic toxin regulates thermophilicity in the crenarchaeote Sulfolobus solfataricus.

5. Improved reactivity of hepatitis C virus core protein epitopes in a conformational antigen-presenting system.

6. ModA and ModB, two ADP-ribosyltransferases encoded by bacteriophage T4: catalytic properties and mutation analysis.

7. Folding and aggregation of TEM beta-lactamase: analogies with the formation of inclusion bodies in Escherichia coli.

Review 8. Strategies for achieving high-level expression of genes in Escherichia coli.

9. Physiological consequences of DnaK and DnaJ overproduction in Escherichia coli.

10. Overexpression of bacterial hemoglobin causes incorporation of pre-beta-lactamase into cytoplasmic inclusion bodies.