Literature DB >> 1369398

Functional expression of GABAA chloride channels and benzodiazepine binding sites in baculovirus infected insect cells.

D B Carter1, D R Thomsen, W B Im, D J Lennon, D M Ngo, W Gale, H K Im, P H Seeburg, M W Smith.   

Abstract

We have employed the baculovirus expression system for the production of insect cell membranes having GABA/benzodiazepine binding sites. Three recombinant baculoviruses each harboring a different GABAA receptor cDNA were introduced into insect cells by simultaneous infection. Infected cells expressed GABA responsive Cl- channels and benzodiazepine binding sites with the same pharmacological specificity as animal cells expressing these receptor subunits.

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Year:  1992        PMID： 1369398     DOI： 10.1038/nbt0692-679

Source DB:  PubMed          Journal:  Biotechnology (N Y)        ISSN： 0733-222X


  9 in total

1.  Characterization of U-97775 as a GABAA receptor ligand of dual functionality in cloned rat GABAA receptor subtypes.

Authors:  H K Im; W B Im; J F Pregenzer; D B Carter; E J Jacobsen; B J Hamilton
Journal:  Br J Pharmacol       Date:  1995-05       Impact factor: 8.739

2.  Expression of a neuronal nicotinic acetylcholine receptor in insect and mammalian host cell systems.

Authors:  E M Aztiria; M C Sogayar; F J Barrantes
Journal:  Neurochem Res       Date:  2000-01       Impact factor: 3.996

3.  Rapid desensitization of alpha 1 beta 1 GABA A receptors expressed in Sf9 cells under optimized conditions.

Authors:  B Birnir; M L Tierney; N P Pillai; G B Cox; P W Gage
Journal:  J Membr Biol       Date:  1995-11       Impact factor: 1.843

4.  A point mutation in the gamma2 subunit of gamma-aminobutyric acid type A receptors results in altered benzodiazepine binding site specificity.

Authors:  A Buhr; E Sigel
Journal:  Proc Natl Acad Sci U S A       Date:  1997-08-05       Impact factor: 11.205

5.  Fragment of GABA(A) receptor containing key ligand-binding residues overexpressed in Escherichia coli.

Authors:  H Xue; R Chu; J Hang; P Lee; H Zheng
Journal:  Protein Sci       Date:  1998-01       Impact factor: 6.725

6.  Differential potentiation of GABAA receptor function by two stereoisomers of diimidazoquinazoline analogues.

Authors:  H K Im; W B Im; J F Pregenzer; J D Petke; B J Hamilton; D B Carter; P F Von Voigtlander; H C Hansen; M Kristiansen
Journal:  Br J Pharmacol       Date:  1992-10       Impact factor: 8.739

7.  Effects of GABA and various allosteric ligands on TBPS binding to cloned rat GABA(A) receptor subtypes.

Authors:  W B Im; J F Pregenzer; D R Thomsen
Journal:  Br J Pharmacol       Date:  1994-08       Impact factor: 8.739

8.  Differential affinity of dihydroimidazoquinoxalines and diimidazoquinazolines to the alpha 1 beta 2 gamma 2 and alpha 6 beta 2 gamma 2 subtypes of cloned GABAA receptors.

Authors:  W B Im; H K Im; J F Pregenzer; B J Hamilton; D B Carter; E J Jacobsen; R E TenBrink; P F VonVoigtlander
Journal:  Br J Pharmacol       Date:  1993-10       Impact factor: 8.739

9.  Expression and purification of a functional heteromeric GABAA receptor for structural studies.

Authors:  Derek P Claxton; Eric Gouaux
Journal:  PLoS One       Date:  2018-07-20       Impact factor: 3.240
  9 in total

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