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Literature DB >> 1369261

Solubility as a function of protein structure and solvent components.

Abstract

This review deals with ways of stabilizing proteins against aggregation and with methods to determine, predict, and increase solubility. Solvent additives (osmolytes) that stabilize proteins are listed with a description of their effects on proteins and on the solvation properties of water. Special attention is given to areas where solubility limitations pose major problems, as in the preparation of highly concentrated solutions of recombinant proteins for structural determination with NMR and X-ray crystallography, refolding of inclusion body proteins, studies of membrane protein dynamics, and in the formulation of proteins for pharmaceutical use. Structural factors relating to solubility and possibilities for protein engineering are analyzed.

Entities: Chemical Disease

Mesh：

Substances：

Year: 1990 PMID： 1369261 DOI： 10.1038/nbt0490-308

Source DB: PubMed Journal: Biotechnology (N Y) ISSN： 0733-222X

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Cited

41 in total

Review 1. Antagonists to the rescue.

Authors: W J Welch; M Howard
Journal: J Clin Invest Date: 2000-04 Impact factor: 14.808

2. Extracellular and cellular distribution of muramidase-2 and muramidase-1 of Enterococcus hirae ATCC 9790.

Authors: R Kariyama; G D Shockman
Journal: J Bacteriol Date: 1992-05 Impact factor: 3.490

3. Amino acid contribution to protein solubility: Asp, Glu, and Ser contribute more favorably than the other hydrophilic amino acids in RNase Sa.

Authors: Saul R Trevino; J Martin Scholtz; C Nick Pace
Journal: J Mol Biol Date: 2006-10-13 Impact factor: 5.469

4. The button test: a small scale method using microdialysis cells for assessing protein solubility at concentrations suitable for NMR.

Authors: S Bagby; K I Tong; D Liu; J R Alattia; M Ikura
Journal: J Biomol NMR Date: 1997-10 Impact factor: 2.835

10. Sorbitol counteracts temperature- and chemical-induced denaturation of a recombinant α-amylase from alkaliphilic Bacillus sp. TS-23.

Authors: Meng-Chun Chi; Tai-Jung Wu; Hsing-Ling Chen; Huei-Fen Lo; Long-Liu Lin
Journal: J Ind Microbiol Biotechnol Date: 2012-08-12 Impact factor: 3.346

Solubility as a function of protein structure and solvent components.

Review 1. Antagonists to the rescue.

2. Extracellular and cellular distribution of muramidase-2 and muramidase-1 of Enterococcus hirae ATCC 9790.

3. Amino acid contribution to protein solubility: Asp, Glu, and Ser contribute more favorably than the other hydrophilic amino acids in RNase Sa.

4. The button test: a small scale method using microdialysis cells for assessing protein solubility at concentrations suitable for NMR.

5. Correcting temperature-sensitive protein folding defects.

6. Osmolytic Effect of Sucrose on Thermal Denaturation of Pea Seedling Copper Amine Oxidase.

7. Guiding protein aggregation with macromolecular crowding.

8. Spectrofluorimetric assessment of the surface hydrophobicity of proteins.

9. Expressing gK gene of duck enteritis virus guided by bioinformatics and its applied prospect in diagnosis.

10. Sorbitol counteracts temperature- and chemical-induced denaturation of a recombinant α-amylase from alkaliphilic Bacillus sp. TS-23.