Literature DB >> 1369106

Chemically defined medium for the production of biologically active substances of CHO cells.

J Hata1, T Tamura, S Yokoshima, S Yamashita, S Kabeno, K Matsumoto, K Onodera.   

Abstract

A recombinant CHO cell line (GT19) secreting a high level of human growth hormone (hGH) was constructed with amplification of the introduced hGH gene. The cells grew well in the alpha MEM medium supplemented with 5% dialyzed fetal calf serum (dFCS), but not with less than 1% dFCS. Therefore we examined various medium components and obtained an improved medium which supported cell growth at low serum concentrations. The production of hGH by the cells was also enhanced in this medium.

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Year:  1992        PMID： 1369106     DOI： 10.1007/bf00376095

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  12 in total

1.  Two types of ribosome in mouse-hamster hybrid cells.

Authors:  C P Stanners; G L Eliceiri; H Green
Journal:  Nat New Biol       Date:  1971-03-10

2.  Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.

Authors:  F T Kao; T T Puck
Journal:  Proc Natl Acad Sci U S A       Date:  1968-08       Impact factor: 11.205

3.  Long-term multiplication of the Chinese hamster ovary (CHO) cell line in a serum-free medium.

Authors:  F Gasser; P Mulsant; M Gillois
Journal:  In Vitro Cell Dev Biol       Date:  1985-10

4.  Human growth hormone as a reporter gene in regulation studies employing transient gene expression.

Authors:  R F Selden; K B Howie; M E Rowe; H M Goodman; D D Moore
Journal:  Mol Cell Biol       Date:  1986-09       Impact factor: 4.272

5.  Highly inducible expression from vectors containing multiple GRE's in CHO cells overexpressing the glucocorticoid receptor.

Authors:  D I Israel; R J Kaufman
Journal:  Nucleic Acids Res       Date:  1989-06-26       Impact factor: 16.971

6.  Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.

Authors:  H Potter; L Weir; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

7.  Constitutive long-term production and characterization of recombinant human interferon-gammas from two different mammalian cells.

Authors:  E Sano; K Okano; R Sawada; M Naruto; T Sudo; K Kamata; M Iizuka; S Kobayashi
Journal:  Cell Struct Funct       Date:  1988-04       Impact factor: 2.212

8.  Conditional thymidine auxotrophic mutants of mouse FM3A cells due to thermosensitive thymidylate synthase and their prototrophic revertants.

Authors:  D Ayusawa; K Iwata; T Seno; H Koyama
Journal:  J Biol Chem       Date:  1981-12-10       Impact factor: 5.157

9.  Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity.

Authors:  G Urlaub; L A Chasin
Journal:  Proc Natl Acad Sci U S A       Date:  1980-07       Impact factor: 11.205

10.  Clonal growth of chinese hamster cell lines in protein-free media.

Authors:  W G Hamilton; R G Ham
Journal:  In Vitro       Date:  1977-09
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  1 in total

1.  A new culture system for the cultivation of mammalian cells for the production of several biologically active substances.

Authors:  M Murata; M Togami; Y Tawara; K Onodera
Journal:  Cytotechnology       Date:  1993       Impact factor: 2.058
  1 in total

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