Literature DB >> 1368888

Single tree genetic linkage mapping in conifers using haploid DNA from megagametophytes.

L K Tulsieram1, J C Glaubitz, G Kiss, J E Carlson.   

Abstract

We report a unique application of the Random Amplified Polymorphic DNA (RAPD) technique for genetic linkage mapping of a single spruce tree using haploid DNA from megagametophyte tissue of individual seeds. Sixty-one segregating loci were analysed for reproducibility, inheritance and linkage. Forty-seven of the 61 markers were distributed into 12 linkage groups and covered 873.8 cM. The 14 markers not associated with any linkage group should be assigned to linkage groups as more markers are added to the map. This new approach quickly provides molecular genetic markers that are simple to evaluate for constructing genetic linkage maps and for other related genetic studies in forest tree species.

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Year:  1992        PMID： 1368888     DOI： 10.1038/nbt0692-686

Source DB:  PubMed          Journal:  Biotechnology (N Y)        ISSN： 0733-222X


  44 in total

1.  Mapping quantitative trait loci by genotyping haploid tissues.

Authors:  R L Wu
Journal:  Genetics       Date:  1999-08       Impact factor: 4.562

2.  Genetic linkage map of the edible basidiomycete Pleurotus ostreatus.

Authors:  L M Larraya; G Pérez; E Ritter; A G Pisabarro; L Ramírez
Journal:  Appl Environ Microbiol       Date:  2000-12       Impact factor: 4.792

3.  A composite linkage map from two crosses for the species complex Picea mariana x Picea rubens and analysis of synteny with other Pinaceae.

Authors:  Betty Pelgas; Jean Bousquet; Stéphanie Beauseigle; Nathalie Isabel
Journal:  Theor Appl Genet       Date:  2005-11-10       Impact factor: 5.699

4.  Constructing the parental linkage phase and the genetic map over distances <1 cM using pooled haploid DNA.

Authors:  Dario Gasbarra; Mikko J Sillanpää
Journal:  Genetics       Date:  2005-11-19       Impact factor: 4.562

5.  Molecular genetics of growth and development in Populus. III. A genetic linkage map of a hybrid poplar composed of RFLP, STS, and RAPD markers.

Authors:  H D Bradshaw; M Villar; B D Watson; K G Otto; S Stewart; R F Stettler
Journal:  Theor Appl Genet       Date:  1994-10       Impact factor: 5.699

6.  An integrated genetic linkage map for eucalypts using RFLP, RAPD and isozyme markers.

Authors:  M Byrne; J C Murrell; B Allen; G F Moran
Journal:  Theor Appl Genet       Date:  1995-11       Impact factor: 5.699

7.  A comparison of RAPD and isozyme analyses for determining the genetic relationships among Avena sterilis L. accessions.

Authors:  M Heun; J P Murphy; T D Phillips
Journal:  Theor Appl Genet       Date:  1994-01       Impact factor: 5.699

8.  Random-amplified-polymorphic DNA markers in sorghum.

Authors:  S Pammi; K Schertz; G Xu; G Hart; J E Mullet
Journal:  Theor Appl Genet       Date:  1994-09       Impact factor: 5.699

9.  Random amplified polymorphic DNA markers tightly linked to a gene for resistance to white pine blister rust in sugar pine.

Authors:  M E Devey; A Delfino-Mix; B B Kinloch; D B Neale
Journal:  Proc Natl Acad Sci U S A       Date:  1995-03-14       Impact factor: 11.205

10.  A diverse family of phenylalanine ammonia-lyase genes expressed in pine trees and cell cultures.

Authors:  S L Butland; M L Chow; B E Ellis
Journal:  Plant Mol Biol       Date:  1998-05       Impact factor: 4.076
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