Literature DB >> 1368816

An easy-to-handle semi-automated method for media development using a colorimetric viability assay and fractional factorial designs.

P Rexen1, J V Kierulff, C Emborg.   

Abstract

A rapid and effective semi-automated screening method has been developed for the development of growth media for mammalian cell culture. The method has proven to be a powerful tool for preliminary evaluation and comparison of new media formulations, but has some inherent disadvantages, which are important to recognise in order to interpret the results.

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Year:  1992        PMID： 1368816     DOI： 10.1007/bf02522036

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  9 in total

1.  Hybridoma growth and monoclonal antibody production in iron-rich protein-free medium: effect of nutrient concentration.

Authors:  F Franĕk; J Dolníková
Journal:  Cytotechnology       Date:  1991-09       Impact factor: 2.058

2.  Agitation and aeration effects in suspension mammalian cell cultures.

Authors:  C P Marquis; K S Low; J P Barford; C Harbour
Journal:  Cytotechnology       Date:  1989-08       Impact factor: 2.058

3.  Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.

Authors:  M C Alley; D A Scudiero; A Monks; M L Hursey; M J Czerwinski; D L Fine; B J Abbott; J G Mayo; R H Shoemaker; M R Boyd
Journal:  Cancer Res       Date:  1988-02-01       Impact factor: 12.701

4.  Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill.

Authors:  M B Hansen; S E Nielsen; K Berg
Journal:  J Immunol Methods       Date:  1989-05-12       Impact factor: 2.303

5.  Use of MTT colorimetric assay to measure cell activation.

Authors:  D Gerlier; N Thomasset
Journal:  J Immunol Methods       Date:  1986-11-20       Impact factor: 2.303

6.  Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.

Authors:  T Mosmann
Journal:  J Immunol Methods       Date:  1983-12-16       Impact factor: 2.303

Review 7.  Tetrazolium salts and formazans.

Authors:  F P Altman
Journal:  Prog Histochem Cytochem       Date:  1976

8.  Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability.

Authors:  F Denizot; R Lang
Journal:  J Immunol Methods       Date:  1986-05-22       Impact factor: 2.303

9.  A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity.

Authors:  P R Twentyman; M Luscombe
Journal:  Br J Cancer       Date:  1987-09       Impact factor: 7.640
  9 in total
  2 in total

1.  Identification of media supplements that improve the viability of primarily cell cultures of Crassostrea gigas oysters.

Authors:  I Domart-Coulon; D Doumenc; S Auzoux-Bordenave; Y Le Fichant
Journal:  Cytotechnology       Date:  1994       Impact factor: 2.058

2.  A multiwell plate-based system for toxicity screening under multiple static or cycling oxygen environments.

Authors:  Ming Yao; Glenn Walker; Michael P Gamcsik
Journal:  Sci Rep       Date:  2021-02-17       Impact factor: 4.379
  2 in total

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