Literature DB >> 1368624

Utilization and stability of vitamins in serum-containing and serum-free media in CHO cell culture.

S Kurano1, N Kurano, C Leist, A Fiechter.   

Abstract

The concentrations of four vitamins, ascorbic acid, nicotinamide, choline and thiamine were evaluated in the culture supernatant of Chinese hamster ovary (CHO) cells. The media used were alpha-modified Eagle's minimum essential medium (MEM-alpha) supplemented with 10% fetal calf serum, and a 1:1 mixture of Ham's F12 and Dulbecco's modified Eagle's medium (DME/F12), containing neither serum nor protein. The reference experiment without cells revealed instability of ascorbic acid and thiamine. Moreover, a significant amount of each vitamin decreased in the culture supernatant. The possibility of growth limitation by vitamin depletion is strongly suggested.

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Year:  1990        PMID： 1368624     DOI： 10.1007/bf00563784

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  12 in total

1.  A fluorimetric method for quantitation in the picomole range of N1-methylnicotinamide and nicotinamide in serum.

Authors:  B R Clark; R M Halpern; R A Smith
Journal:  Anal Biochem       Date:  1975-09       Impact factor: 3.365

2.  Growth kinetics of Chinese hamster ovary cells in a compact loop bioreactor. 3. Selection and characterization of an anchorage-independent subline and medium improvement.

Authors:  N Kurano; C Leist; F Messi; C Gandor; S Kurano; A Fiechter
Journal:  J Biotechnol       Date:  1990-11       Impact factor: 3.307

3.  Spin filter perfusion system for high density cell culture: production of recombinant urinary type plasminogen activator in CHO cells.

Authors:  G C Avgerinos; D Drapeau; J S Socolow; J I Mao; K Hsiao; R J Broeze
Journal:  Biotechnology (N Y)       Date:  1990-01

4.  Growth behavior of Chinese hamster ovary cells in a compact loop bioreactor: 1. Effects of physical and chemical environments.

Authors:  N Kurano; C Leist; F Messi; S Kurano; A Fiechter
Journal:  J Biotechnol       Date:  1990-07       Impact factor: 3.307

5.  Fluorometric enzyme assay for choline and acetylcholine.

Authors:  E T Browning
Journal:  Anal Biochem       Date:  1972-04       Impact factor: 3.365

6.  Determination of intracellular choline levels by an enzymatic assay.

Authors:  M K Hise; C M Mansbach
Journal:  Anal Biochem       Date:  1983-11       Impact factor: 3.365

7.  Separation of choline- and ethanolamine-labeled metabolites by ion-exchange high-pressure liquid chromatography.

Authors:  G Mazzola; C Kent
Journal:  Anal Biochem       Date:  1984-08-15       Impact factor: 3.365

8.  Determination of picomole quantities of acetylcholine and choline in physiologic salt solutions.

Authors:  M L Gilberstadt; J A Russell
Journal:  Anal Biochem       Date:  1984-04       Impact factor: 3.365

9.  Estimation of N-amino-N,N-dimethylaminoethanol, choline and their acetate esters by gas chromatography mass spectrometry.

Authors:  M W Newton; B Ringdahl; D J Jenden
Journal:  Anal Biochem       Date:  1983-04-01       Impact factor: 3.365

10.  Growth behavior of Chinese hamster ovary cells in a compact loop bioreactor. 2. Effects of medium components and waste products.

Authors:  N Kurano; C Leist; F Messi; S Kurano; A Fiechter
Journal:  J Biotechnol       Date:  1990-07       Impact factor: 3.307
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  2 in total

1.  The role of vitamins and amino acids on hybridoma growth and monoclonal antibody production.

Authors:  P Ducommun; P A Ruffieux; U von Stockar; I Marison
Journal:  Cytotechnology       Date:  2001-10       Impact factor: 2.058

Review 2.  Oxidative stress-alleviating strategies to improve recombinant protein production in CHO cells.

Authors:  Valentine Chevallier; Mikael Rørdam Andersen; Laetitia Malphettes
Journal:  Biotechnol Bioeng       Date:  2019-12-20       Impact factor: 4.530
  2 in total

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