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Literature DB >> 1368596

A novel isolation method for hen egg yolk antibody, "IgY".

Abstract

A method for isolation of egg yolk immunoglobulin, IgG, a livetin protein, was investigated. Several natural gums (carrageenan and xanthan gum) were found to be effective for removal of yolk lipoprotein as a precipitate. The effect was pronounced with lambda-carrageenan and the lipid content in the supernatant after removal of the resulting precipitate was less than 0.4% of that of egg yolk. IgY remained in this supernatant, with a yield of 86%, and was isolated by chromatography on a column of DEAE-Sephacel followed by salting-out with sodium sulfate. IgY thus isolated was almost pure (98%) and the yield was 70 to 100 mg per egg.

Entities: Chemical

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Year: 1990 PMID： 1368596

Source DB: PubMed Journal: Agric Biol Chem ISSN： 0002-1369

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Cited

12 in total

1. Antibodies against Venom of the Snake Deinagkistrodon acutus.

Authors: Chi-Hsin Lee; Yu-Ching Lee; Meng-Huei Liang; Sy-Jye Leu; Liang-Tzung Lin; Jen-Ron Chiang; Yi-Yuan Yang
Journal: Appl Environ Microbiol Date: 2015-10-16 Impact factor: 4.792

2. Structures of asparagine-linked oligosaccharides from hen egg-yolk antibody (IgY). Occurrence of unusual glucosylated oligo-mannose type oligosaccharides in a mature glycoprotein.

Authors: M Ohta; J Hamako; S Yamamoto; H Hatta; M Kim; T Yamamoto; S Oka; T Mizuochi; F Matsuura
Journal: Glycoconj J Date: 1991-10 Impact factor: 2.916

3. Structures of asparagine linked oligosaccharides of immunoglobulins (IgY) isolated from egg-yolk of Japanese quail.

Authors: F Matsuura; M Ohta; K Murakami; Y Matsuki
Journal: Glycoconj J Date: 1993-06 Impact factor: 2.916

4. Immunology of the pathogen virulence and phytotoxin production in relation to disease severity: a case study in sheath blight of rice.

Authors: V Shanmugam; R Viswanathan; T Raguchander; P Balasubramanian; R Samiyappan
Journal: Folia Microbiol (Praha) Date: 2002 Impact factor: 2.099

5. Oral passive immunization against dental caries in rats by use of hen egg yolk antibodies specific for cell-associated glucosyltransferase of Streptococcus mutans.

Authors: S Hamada; T Horikoshi; T Minami; S Kawabata; J Hiraoka; T Fujiwara; T Ooshima
Journal: Infect Immun Date: 1991-11 Impact factor: 3.441

6. Development of Indirect Competitive ELISA for Lithospermic Acid B of Salvia miltiorrhiza with Its Specific Antibodies Generated via Artificial Oil Bodies.

Authors: Yu-En Shih; Chao-Hsiang Chen; Nan-Hei Lin; Jason T C Tzen
Journal: Molecules Date: 2019-05-21 Impact factor: 4.927

A novel isolation method for hen egg yolk antibody, "IgY".

1. Antibodies against Venom of the Snake Deinagkistrodon acutus.

2. Structures of asparagine-linked oligosaccharides from hen egg-yolk antibody (IgY). Occurrence of unusual glucosylated oligo-mannose type oligosaccharides in a mature glycoprotein.

3. Structures of asparagine linked oligosaccharides of immunoglobulins (IgY) isolated from egg-yolk of Japanese quail.

4. Immunology of the pathogen virulence and phytotoxin production in relation to disease severity: a case study in sheath blight of rice.

5. Oral passive immunization against dental caries in rats by use of hen egg yolk antibodies specific for cell-associated glucosyltransferase of Streptococcus mutans.

6. Development of Indirect Competitive ELISA for Lithospermic Acid B of Salvia miltiorrhiza with Its Specific Antibodies Generated via Artificial Oil Bodies.

7. Application of an immunoglobulin Y-alkaline phosphatase bioconjugate as a diagnostic tool for influenza A virus.

8. Simple and efficient protocol for immunoglobulin Y purification from chicken egg yolk.

9. The preparation of N-IgY targeting SARS-CoV-2 and its immunomodulation to IFN-γ production in vitro.

10. Successful treatment of rotavirus-induced diarrhoea in suckling mice with egg yolk immunoglobulin.