Literature DB >> 1368564

Cloning and expression of the creatinase gene from Flavobacterium sp. U-188 in Escherichia coli.

Y Koyama1, S Kitao, H Yamamoto-Otake, M Suzuki, E Nakano.   

Abstract

The gene coding for creatinase (creatine amidinohydrolase, EC 3.5.3.3) was isolated from Flavobacterium sp. U-188. The primary structure of creatinase deduced from the nucleotide sequence showed a protein (molecular weight, 42, 651) composed of 378 amino acids. The creatinase gene was over-expressed in Escherichia coli under the control of the lac promoter and the amount of this enzyme was over 20% of the soluble protein in the cell.

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Year:  1990        PMID： 1368564

Source DB:  PubMed          Journal:  Agric Biol Chem        ISSN： 0002-1369


  8 in total

1.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1991-01-11       Impact factor: 16.971

2.  Unusually biased nucleotide sequences on sense strands of Flavobacterium sp. genes produce nonstop frames on the corresponding antisense strands.

Authors:  K Ikehara; E Okazawa
Journal:  Nucleic Acids Res       Date:  1993-05-11       Impact factor: 16.971

3.  Sequence and structure comparison suggest that methionine aminopeptidase, prolidase, aminopeptidase P, and creatinase share a common fold.

Authors:  J F Bazan; L H Weaver; S L Roderick; R Huber; B W Matthews
Journal:  Proc Natl Acad Sci U S A       Date:  1994-03-29       Impact factor: 11.205

4.  Production of novel NaN3-resistant creatine amidinohydrolase in recombinant Escherichia coli.

Authors:  Song Liu; Jun Dai; Zhen Kang; Jianghua Li; Jian Chen; Guocheng Du
Journal:  Bioengineered       Date:  2015       Impact factor: 3.269

5.  Cloning, sequencing, and expression of the N-acyl-D-mannosamine dehydrogenase gene from Flavobacterium sp. strain 141-8 in Escherichia coli.

Authors:  H Yamamoto-Otake; Y Koyama; T Horiuchi; E Nakano
Journal:  Appl Environ Microbiol       Date:  1991-05       Impact factor: 4.792

6.  Cloning of a creatinase gene from Pseudomonas putida in Escherichia coli by using an indicator plate.

Authors:  M C Chang; C C Chang; J C Chang
Journal:  Appl Environ Microbiol       Date:  1992-10       Impact factor: 4.792

7.  Cloning, Expression and Purification of Pseudomonas putida ATCC12633 Creatinase.

Authors:  Elnaz Afshari; Zahra Amini-Bayat; Saman Hosseinkhani; Nahid Bakhtiari
Journal:  Avicenna J Med Biotechnol       Date:  2017 Oct-Dec

8.  Improved thermostability of creatinase from Alcaligenes Faecalis through non-biased phylogenetic consensus-guided mutagenesis.

Authors:  Xue Bai; Daixi Li; Fuqiang Ma; Xi Deng; Manjie Luo; Yan Feng; Guangyu Yang
Journal:  Microb Cell Fact       Date:  2020-10-17       Impact factor: 5.328
  8 in total

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