Literature DB >> 1368506

Selective determination of L-lysine with L-lysine epsilon-dehydrogenase.

H Hashimoto1, H Misono, S Nagata, S Nagasaki.   

Abstract

Simple and sensitive spectrophotometric methods were developed for the selective determination of L-lysine. The assay methods were based on the spectrophotometric determination of NADH formed in the reaction catalyzed by L-lysine epsilon-dehydrogenase (Procedure A) or the formazan produced on coupling of the dehydrogenase reaction with the reduction of a tetrazolium salt (Procedure B). Procedure B was applicable to the direct determination of L-lysine in rabbit serum.

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Year:  1990        PMID： 1368506

Source DB:  PubMed          Journal:  Agric Biol Chem        ISSN： 0002-1369


  3 in total

1.  E. coli biosensor based on modular GFP and luxI/luxR cyclic amplification circuit for sensitive detection of lysine.

Authors:  Wenjia Wang; Jing Zhang; Hui Tao; Xuefei Lv; Yulin Deng; Xiaoqiong Li
Journal:  Anal Bioanal Chem       Date:  2022-10-18       Impact factor: 4.478

2.  First crystal structure of L-lysine 6-dehydrogenase as an NAD-dependent amine dehydrogenase.

Authors:  Kazunari Yoneda; Junya Fukuda; Haruhiko Sakuraba; Toshihisa Ohshima
Journal:  J Biol Chem       Date:  2010-01-07       Impact factor: 5.157

3.  Highly stable L-lysine 6-dehydrogenase from the thermophile Geobacillus stearothermophilus isolated from a Japanese hot spring: characterization, gene cloning and sequencing, and expression.

Authors:  Mojgan Heydari; Toshihisa Ohshima; Naoki Nunoura-Kominato; Haruhiko Sakuraba
Journal:  Appl Environ Microbiol       Date:  2004-02       Impact factor: 4.792
  3 in total

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