Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Use of fluorescence anisotropy determinations for indicating the physiological status of hybridoma cell cultures.

Literature DB >> 1368404

Use of fluorescence anisotropy determinations for indicating the physiological status of hybridoma cell cultures.

V Eyl¹, S Muller, M Donner, M Maugras, J F Stoltz.

Abstract

The evolution of lipid compartment fluidity during culture of hybridoma cells was studied by fluorescence polarization measurements. The probe partition between the plasma membrane and intracytoplasmic compartments was determined by a quenching fluorescence method. A progressive decrease of the plasma membrane fluidity was observed during the growth phase with an increase during stationary and degeneration phases of the culture. These data suggest that fluidity parameters could be used to follow the behaviour of hybridoma cell cultures.

Entities: Chemical

Mesh：

Substances：

Year: 1992 PMID： 1368404 DOI： 10.1007/bf02540024

Source DB: PubMed Journal: Cytotechnology ISSN： 0920-9069 Impact factor: 2.058

Keyword Cloud
References

14 in total

1. The distribution of 1,6 diphenyl hexatriene fluorescence in normal human lymphocytes.

Authors: S M Johnson; C Nicolau
Journal: Biochem Biophys Res Commun Date: 1977-06-06 Impact factor: 3.575

2. Intracellular distribution of lipophilic fluorescent probes in mammalian cells.

Authors: R E Pagano; K Ozato; J M Ruysschaert
Journal: Biochim Biophys Acta Date: 1977-03-17

3. Correlation between cell density, membrane fluidity, and the availability of transferrin receptors in Friend erythroleukemic cells.

Authors: C P Muller; Z Volloch; M Shinitzky
Journal: Cell Biophys Date: 1980-09

4. Effects of differentiation inducers on diphenylhexatriene fluorescence polarization in intracytoplasmic and plasma membranes from Friend erythroleukemia cells.

Authors: C Billard; M Billard; Z Mishal
Journal: Biochem Biophys Res Commun Date: 1983-11-30 Impact factor: 3.575

Review 5. Molecular biology of cell activation.

Authors: L Kaczmarek; B Kamińska
Journal: Exp Cell Res Date: 1989-07 Impact factor: 3.905

6. The plasma-membrane internalization and recycling is enhanced in macrophages upon activation with gamma-interferon and lipopolysaccharide; a study using the fluorescent probe trimethylaminodiphenylhexatriene.

Authors: D Illinger; P Poindron; N Glasser; M Modollel; J G Kuhry
Journal: Biochim Biophys Acta Date: 1990-11-30

Use of fluorescence anisotropy determinations for indicating the physiological status of hybridoma cell cultures.

1. The distribution of 1,6 diphenyl hexatriene fluorescence in normal human lymphocytes.

2. Intracellular distribution of lipophilic fluorescent probes in mammalian cells.

3. Correlation between cell density, membrane fluidity, and the availability of transferrin receptors in Friend erythroleukemic cells.

4. Effects of differentiation inducers on diphenylhexatriene fluorescence polarization in intracytoplasmic and plasma membranes from Friend erythroleukemia cells.

Review 5. Molecular biology of cell activation.

6. The plasma-membrane internalization and recycling is enhanced in macrophages upon activation with gamma-interferon and lipopolysaccharide; a study using the fluorescent probe trimethylaminodiphenylhexatriene.

7. Inhibition of growth and metastases in mice by immunization with cholesterol hemisuccinate-enriched tumor cells.

8. Membrane dynamics of Friend leukaemic cells. I. Changes associated with cell growth.

9. Localization of the lipid probe 1,6-diphenyl-1,3,5 hexatriene (DPH) in intact cells by fluorescence microscopy.

10. Evolution of fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) during the labelling of living cells.