| Literature DB >> 1367984 |
N Tonouchi1, N Koyama, K Miwa.
Abstract
A Chinese Hamster Ovary (CHO) strain producing high-level human interleukin 6 (IL-6) was obtained, by introduction of the IL-6 cDNA from which the 3' AU-rich region was deleted. The IL-6 mRNA of this strain was stable. The productivity was more than 15 times higher than the previously obtained clone, which has intact IL-6 cDNA in the same expression vector. This strain produced IL-6 at a high level of 30 micrograms ml-1 in batch culture, or a continuous 20 micrograms per 10(6) cells per day in microcarrier cultivation.Entities:
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Year: 1992 PMID： 1367984 DOI： 10.1016/0168-1656(92)90146-z
Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307