Literature DB >> 1367516

Saccharomyces cerevisiae strains that overexpress heterologous proteins.

D Sleep1, G P Belfield, D J Ballance, J Steven, S Jones, L R Evans, P D Moir, A R Goodey.   

Abstract

We describe a system that facilitates the selection of host mutants that overproduce a range of secreted and internally produced heterologous proteins in Saccharomyces cerevisiae. These mutants were initially selected for their ability to oversecrete recombinant human albumin (rHA), as detected by a direct visual assay that relies upon antibody precipitation in solid media. Yeast strains that were able to synthesize and secrete increased levels of rHA also produced elevated levels of internally expressed proteins including alpha 1-antitrypsin Pittsburgh variant and plasminogen activator inhibitor type 2.

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Year:  1991        PMID： 1367516     DOI： 10.1038/nbt0291-183

Source DB:  PubMed          Journal:  Biotechnology (N Y)        ISSN： 0733-222X


  11 in total

1.  Transferrin fusion technology: a novel approach to prolonging biological half-life of insulinotropic peptides.

Authors:  Byung-Joon Kim; Jie Zhou; Bronwen Martin; Olga D Carlson; Stuart Maudsley; Nigel H Greig; Mark P Mattson; Ellen E Ladenheim; Jay Wustner; Andrew Turner; Homayoun Sadeghi; Josephine M Egan
Journal:  J Pharmacol Exp Ther       Date:  2010-05-24       Impact factor: 4.030

2.  Interdomain zinc site on human albumin.

Authors:  Alan J Stewart; Claudia A Blindauer; Stephen Berezenko; Darrell Sleep; Peter J Sadler
Journal:  Proc Natl Acad Sci U S A       Date:  2003-02-21       Impact factor: 11.205

3.  Direct screening for high-level expression of an introduced alpha-amylase gene in plants.

Authors:  J Pen; A J van Ooyen; P J van den Elzen; K Rietveld; A Hoekema
Journal:  Plant Mol Biol       Date:  1992-04       Impact factor: 4.076

4.  Use of the KlADH4 promoter for ethanol-dependent production of recombinant human serum albumin in Kluyveromyces lactis.

Authors:  M Saliola; C Mazzoni; N Solimando; A Crisà; C Falcone; G Jung; R Fleer
Journal:  Appl Environ Microbiol       Date:  1999-01       Impact factor: 4.792

5.  Use of Aspergillus overproducing mutants, cured for integrated plasmid, to overproduce heterologous proteins.

Authors:  M Ward; L J Wilson; K H Kodama
Journal:  Appl Microbiol Biotechnol       Date:  1993-08       Impact factor: 4.813

6.  Modulation of chaperone gene expression in mutagenized Saccharomyces cerevisiae strains developed for recombinant human albumin production results in increased production of multiple heterologous proteins.

Authors:  T Payne; C Finnis; L R Evans; D J Mead; S V Avery; D B Archer; D Sleep
Journal:  Appl Environ Microbiol       Date:  2008-10-17       Impact factor: 4.792

Review 7.  Co-opting biology to deliver drugs.

Authors:  Parisa Yousefpour; Ashutosh Chilkoti
Journal:  Biotechnol Bioeng       Date:  2014-07-21       Impact factor: 4.530

8.  Glycan modification of a thermostable recombinant (1-3, 1-4)-beta-glucanase secreted from Saccharomyces cerevisiae is determined by strain and culture conditions.

Authors:  M Meldgaard; J Harthill; B Petersen; O Olsen
Journal:  Glycoconj J       Date:  1995-06       Impact factor: 2.916

9.  High-level production of animal-free recombinant transferrin from Saccharomyces cerevisiae.

Authors:  Christopher J A Finnis; Tom Payne; Joanna Hay; Neil Dodsworth; Diane Wilkinson; Philip Morton; Malcolm J Saxton; David J Tooth; Robert W Evans; Hans Goldenberg; Barbara Scheiber-Mojdehkar; Nina Ternes; Darrell Sleep
Journal:  Microb Cell Fact       Date:  2010-11-17       Impact factor: 5.328

10.  Structure-based mutagenesis reveals the albumin-binding site of the neonatal Fc receptor.

Authors:  Jan Terje Andersen; Bjørn Dalhus; Jason Cameron; Muluneh Bekele Daba; Andrew Plumridge; Leslie Evans; Stephan O Brennan; Kristin Støen Gunnarsen; Magnar Bjørås; Darrell Sleep; Inger Sandlie
Journal:  Nat Commun       Date:  2012-01-03       Impact factor: 14.919
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