| Literature DB >> 1367468 |
Abstract
Electroporation-mediated transformation of Lactococcus lactis with plasmid pGB301, a 9.8 kilobase pair vector (Behnke et al. 1981), has been reported by McIntyre & Harlander (1989a). Improved transformation efficiencies of 10(2)-10(3)/micrograms DNA were achieved by altering the conditions under which the bacteria were grown prior to electroporation (McIntyre & Harlander 1989b). This present investigation sought to improve still further transformation efficiencies in order to provide a reliable high frequency transformation system for Lc. lactis subsp. lactis.Entities:
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Year: 1990 PMID: 1367468 DOI: 10.1111/j.1472-765x.1990.tb01275.x
Source DB: PubMed Journal: Lett Appl Microbiol ISSN: 0266-8254 Impact factor: 2.858