Literature DB >> 1367468

High efficiency electroporation of Lactococcus lactis subsp. lactis LM0230 with plasmid pGB301.

S Dornan1, M A Collins.   

Abstract

Electroporation-mediated transformation of Lactococcus lactis with plasmid pGB301, a 9.8 kilobase pair vector (Behnke et al. 1981), has been reported by McIntyre & Harlander (1989a). Improved transformation efficiencies of 10(2)-10(3)/micrograms DNA were achieved by altering the conditions under which the bacteria were grown prior to electroporation (McIntyre & Harlander 1989b). This present investigation sought to improve still further transformation efficiencies in order to provide a reliable high frequency transformation system for Lc. lactis subsp. lactis.

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Year:  1990        PMID: 1367468     DOI: 10.1111/j.1472-765x.1990.tb01275.x

Source DB:  PubMed          Journal:  Lett Appl Microbiol        ISSN: 0266-8254            Impact factor:   2.858


  13 in total

1.  The autoproteolysis of Lactococcus lactis lactocepin III affects its specificity towards beta-casein.

Authors:  B Flambard; V Juillard
Journal:  Appl Environ Microbiol       Date:  2000-12       Impact factor: 4.792

2.  Characterization and sequence analysis of a stable cryptic plasmid from Enterococcus faecium 226 and development of a stable cloning vector.

Authors:  H A Wyckoff; M Barnes; K O Gillies; W E Sandine
Journal:  Appl Environ Microbiol       Date:  1996-04       Impact factor: 4.792

3.  The effects of adding lactococcal proteinase on the growth rate of Lactococcus lactis in milk depend on the type of enzyme.

Authors:  S Helinck; J Richard; V Juillard
Journal:  Appl Environ Microbiol       Date:  1997-06       Impact factor: 4.792

4.  Bioencapsulation and Colonization Characteristics of Lactococcus lactis subsp. lactis CF4MRS in Artemia franciscana: a Biological Approach for the Control of Edwardsiellosis in Larviculture.

Authors:  Jiun Yan Loh; Gemma L Kay; Adeline Su Yien Ting
Journal:  Mar Biotechnol (NY)       Date:  2018-04-13       Impact factor: 3.619

5.  The citrate transport system of Lactococcus lactis subsp. lactis biovar diacetylactis is induced by acid stress.

Authors:  N García-Quintáns; C Magni; D de Mendoza; P López
Journal:  Appl Environ Microbiol       Date:  1998-03       Impact factor: 4.792

6.  Contribution of citrate metabolism to the growth of Lactococcus lactis CRL264 at low pH.

Authors:  Claudia Sánchez; Ana Rute Neves; João Cavalheiro; Margarida Moreira dos Santos; Nieves García-Quintáns; Paloma López; Helena Santos
Journal:  Appl Environ Microbiol       Date:  2007-12-21       Impact factor: 4.792

7.  Acid-inducible transcription of the operon encoding the citrate lyase complex of Lactococcus lactis Biovar diacetylactis CRL264.

Authors:  Mauricio G Martín; Pablo D Sender; Salvador Peirú; Diego de Mendoza; Christian Magni
Journal:  J Bacteriol       Date:  2004-09       Impact factor: 3.490

8.  Transcriptional activation of the citrate permease P gene of Lactococcus lactis biovar diacetylactis by an insertion sequence-like element present in plasmid pCIT264.

Authors:  F López de Felipe; C Magni; D de Mendoza; P López
Journal:  Mol Gen Genet       Date:  1996-03-07

9.  Citrate utilization gene cluster of the Lactococcus lactis biovar diacetylactis: organization and regulation of expression.

Authors:  F López de Felipe; C Magni; D de Mendoza; P López
Journal:  Mol Gen Genet       Date:  1995-03-10

10.  High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitol.

Authors:  Maria Papagianni; Nicholaos Avramidis; George Filioussis
Journal:  BMC Biotechnol       Date:  2007-03-21       Impact factor: 2.563

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