Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Monitoring survival and gene transfer in soil microcosms of recombinant Escherichia coli designed to represent an industrial production strain.

Literature DB >> 1367370

Monitoring survival and gene transfer in soil microcosms of recombinant Escherichia coli designed to represent an industrial production strain.

R B Henschke¹, E J Henschke, F R Schmidt.

Abstract

A genetically engineered microorganism (GEM) was designed to exemplify bacterial strains used for the production of biological material in industry. The recombinant DNA was located on a safety plasmid (pUC19). Survival and persistence of the GEM and its recombinant DNA (rDNA) was determined in soil microcosms by using different monitoring methods, including the polymerase-chain reaction, to amplify and detect the specific rDNA. Depending on nutritional status, both the GEM and its rDNA had disappeared within 16 (amended soil) or 28 days (non-amended soil) with a limit of detection of 5 cells/g soil and 20 fg DNA/g soil.

Entities: Species

Mesh：

Substances：

Year: 1991 PMID： 1367370 DOI： 10.1007/bf00184696

Source DB: PubMed Journal: Appl Microbiol Biotechnol ISSN： 0175-7598 Impact factor: 4.813

12 in total

1. DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community.

Authors: William E Holben; Janet K Jansson; Barry K Chelm; James M Tiedje
Journal: Appl Environ Microbiol Date: 1988-03 Impact factor: 4.792

2. Detection of horizontal gene transfer by natural transformation in native and introduced species of bacteria in marine and synthetic sediments.

Authors: G J Stewart; C D Sinigalliano
Journal: Appl Environ Microbiol Date: 1990-06 Impact factor: 4.792

3. Genetic engineering: Processes and products.

Authors: J Davies
Journal: Trends Ecol Evol Date: 1988-04 Impact factor: 17.712

4. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors: R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal: Science Date: 1988-01-29 Impact factor: 47.728

Review 5. The biology of natural transformation.

Authors: G J Stewart; C A Carlson
Journal: Annu Rev Microbiol Date: 1986 Impact factor: 15.500

6. DNA amplification to enhance detection of genetically engineered bacteria in environmental samples.

Authors: R J Steffan; R M Atlas
Journal: Appl Environ Microbiol Date: 1988-09 Impact factor: 4.792

7. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

Authors: J Vieira; J Messing
Journal: Gene Date: 1982-10 Impact factor: 3.688

8. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors: S N Cohen; A C Chang; L Hsu
Journal: Proc Natl Acad Sci U S A Date: 1972-08 Impact factor: 11.205

9. Identification of Frankia strains in nodules by hybridization of polymerase chain reaction products with strain-specific oligonucleotide probes.

Authors: P Simonet; P Normand; A Moiroud; R Bardin
Journal: Arch Microbiol Date: 1990 Impact factor: 2.552

Monitoring survival and gene transfer in soil microcosms of recombinant Escherichia coli designed to represent an industrial production strain.

1. DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community.

2. Detection of horizontal gene transfer by natural transformation in native and introduced species of bacteria in marine and synthetic sediments.

3. Genetic engineering: Processes and products.

4. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Review 5. The biology of natural transformation.

6. DNA amplification to enhance detection of genetically engineered bacteria in environmental samples.

7. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

8. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

9. Identification of Frankia strains in nodules by hybridization of polymerase chain reaction products with strain-specific oligonucleotide probes.

10. Detection of coliform bacteria in water by polymerase chain reaction and gene probes.

Review 1. Bacterial gene transfer by natural genetic transformation in the environment.

2. Validation of Microcosms for Examining the Survival of Pseudomonas aureofaciens (lacZY) in Soil.

3. Kinetics of the persistence of chromosomal DNA from genetically engineered Escherichia coli introduced into soil.

Review 4. Extraction of bacterial RNA from soil: challenges and solutions.