| Literature DB >> 1367359 |
B F McCutchen1, P V Choudary, R Crenshaw, D Maddox, S G Kamita, N Palekar, S Volrath, E Fowler, B D Hammock, S Maeda.
Abstract
Recombinant nuclear polyhedrosis viruses (NPVs) expressing insect-selective toxins, hormones, or enzymes could enhance their insecticidal properties. We have constructed a recombinant, polyhedrin-positive Autographa californica NPV (AcNPV) that is orally infectious and expresses an insect-selective toxin (AaIT), isolated from the scorpion Androctonus australis, under the control of the p10 promoter. Bioassays with the recombinant baculovirus on 2nd instar larvae of Heliothis virescens demonstrated a significant decrease in the time to kill (LT50 88.0 hours) compared to wild-type AcNPV (LT50 125 hours). Production of AaIT was confirmed by western blot analysis of larval hemolymph from infected H. virescens, and bioassays with larvae of Sarcophaga falculata.Entities:
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Year: 1991 PMID： 1367359 DOI： 10.1038/nbt0991-848
Source DB: PubMed Journal: Biotechnology (N Y) ISSN： 0733-222X