| Literature DB >> 1367358 |
P Krimpenfort1, A Rademakers, W Eyestone, A van der Schans, S van den Broek, P Kooiman, E Kootwijk, G Platenburg, F Pieper, R Strijker.
Abstract
We have combined gene transfer, by microinjection, with 'in vitro' embryo production technology, enabling us to carry out non-surgical transfer, to recipient cows, of microinjected embryos that have been cultured from immature oocytes. Using this approach, we have established 21 pregnancies from which 19 calves were born. Southern blot analysis proved that in two cases the microinjected DNA had been integrated in the host genome.Entities:
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Year: 1991 PMID： 1367358 DOI： 10.1038/nbt0991-844
Source DB: PubMed Journal: Biotechnology (N Y) ISSN： 0733-222X