Literature DB >> 1367303

Heterologous gene expression in Hansenula polymorpha: efficient secretion of glucoamylase.

G Gellissen1, Z A Janowicz, A Merckelbach, M Piontek, P Keup, U Weydemann, C P Hollenberg, A W Strasser.   

Abstract

We have introduced the glucoamylase gene (GAM1) from Schwanniomyces occidentalis into the genome of the methylotrophic yeast Hansenula polymorpha to study the potential of this organism as a host for high-level expression of a heterologous gene encoding a secretory protein. Transformants of H. polymorpha containing GAM1 under control of the formate dehydrogenase (FMD) promoter are stable and efficiently secrete an active glucoamylase that is faithfully processed and modified. Yields of up to 1.4g/l of active enzyme were obtained at cell densities of 100-130 grams dry weight per liter.

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Year:  1991        PMID： 1367303     DOI： 10.1038/nbt0391-291

Source DB:  PubMed          Journal:  Biotechnology (N Y)        ISSN： 0733-222X


  22 in total

1.  Exchanging the active site between phytases for altering the functional properties of the enzyme.

Authors:  M Lehmann; R Lopez-Ulibarri; C Loch; C Viarouge; M Wyss; A P van Loon
Journal:  Protein Sci       Date:  2000-10       Impact factor: 6.725

2.  Regulation of the formate dehydrogenase gene, FDH1, in the methylotrophic yeast Candida boidinii and growth characteristics of an FDH1-disrupted strain on methanol, methylamine, and choline.

Authors:  Y Sakai; A P Murdanoto; T Konishi; A Iwamatsu; N Kato
Journal:  J Bacteriol       Date:  1997-07       Impact factor: 3.490

3.  The construction of a stable starch-fermenting yeast strain using genetic engineering and rare-mating.

Authors:  T G Kim; K Kim
Journal:  Appl Biochem Biotechnol       Date:  1996-04       Impact factor: 2.926

4.  Chromosome Polymorphisms among Strains of Hansenula polymorpha (syn. Pichia angusta).

Authors:  L Marri; G M Rossolini; G Satta
Journal:  Appl Environ Microbiol       Date:  1993-03       Impact factor: 4.792

5.  A regulatory mutant of Hansenula polymorpha exhibiting methanol utilization metabolism and peroxisome proliferation in glucose.

Authors:  G Parpinello; E Berardi; R Strabbioli
Journal:  J Bacteriol       Date:  1998-06       Impact factor: 3.490

6.  Purification and properties of methyl formate synthase, a mitochondrial alcohol dehydrogenase, participating in formaldehyde oxidation in methylotrophic yeasts.

Authors:  A P Murdanoto; Y Sakai; T Konishi; F Yasuda; Y Tani; N Kato
Journal:  Appl Environ Microbiol       Date:  1997-05       Impact factor: 4.792

7.  Antibody and cytotoxic T-cell responses to soluble hepatitis B virus (HBV) S antigen in mice: implication for the pathogenesis of HBV-induced hepatitis.

Authors:  R Schirmbeck; K Melber; T Mertens; J Reimann
Journal:  J Virol       Date:  1994-03       Impact factor: 5.103

8.  Directed mutagenesis in an asporogenous methylotrophic yeast: cloning, sequencing, and one-step gene disruption of the 3-isopropylmalate dehydrogenase gene (LEU2) of Candida boidinii to derive doubly auxotrophic marker strains.

Authors:  Y Sakai; Y Tani
Journal:  J Bacteriol       Date:  1992-09       Impact factor: 3.490

9.  Stable multicopy integration of vector sequences in Hansenula polymorpha.

Authors:  R Gatzke; U Weydemann; Z A Janowicz; C P Hollenberg
Journal:  Appl Microbiol Biotechnol       Date:  1995-10       Impact factor: 4.813

10.  Cloning and sequencing of the ura3 locus of the methylotrophic yeast Hansenula polymorpha and its use for the generation of a deletion by gene replacement.

Authors:  A Merckelbach; S Gödecke; Z A Janowicz; C P Hollenberg
Journal:  Appl Microbiol Biotechnol       Date:  1993-11       Impact factor: 4.813
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