Literature DB >> 1366960

A novel, quantitative bioassay for type I interferon using a recombinant indicator cell line.

R Lleonart1, D Näf, H Browning, C Weissmann.   

Abstract

We describe a specific and quantitative novel assay for biologically active human type I interferon (IFN), the MxR assay. It is based on a Vero cell line containing multiple copies of a hybrid gene consisting of the murine Mx promoter, which is responsive to type I IFN, linked to the human growth hormone (hGH) transcription unit. Exposure of this cell line to IFN-alpha or -beta for 12-48 hours results in the production of hGH that is measured by a commercially available radio-immune assay. The response to IFN-alpha is dose-dependent between 3 and 1000 units/ml. There is no response to TNF, IL-1 and a number of other cytokines and growth factors, and only a negligible response to IFN-gamma.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 1366960     DOI： 10.1038/nbt1290-1263

Source DB:  PubMed          Journal:  Biotechnology (N Y)        ISSN： 0733-222X


  6 in total

1.  Species-independent bioassay for sensitive quantification of antiviral type I interferons.

Authors:  Thomas Kuri; Matthias Habjan; Nicola Penski; Friedemann Weber
Journal:  Virol J       Date:  2010-02-26       Impact factor: 4.099

2.  Bioactivity determination of native and variant forms of therapeutic interferons.

Authors:  Louise Larocque; Alex Bliu; Ranran Xu; Abebaw Diress; Junzhi Wang; Rongtuan Lin; Runtao He; Michel Girard; Xuguang Li
Journal:  J Biomed Biotechnol       Date:  2011-03-03

3.  Induced expression of trimerized intracellular domains of the human tumor necrosis factor (TNF) p55 receptor elicits TNF effects.

Authors:  V Vandevoorde; G Haegeman; W Fiers
Journal:  J Cell Biol       Date:  1997-06-30       Impact factor: 10.539

4.  Validation of a cell-based colorimetric reporter gene assay for the evaluation of Type I Interferons.

Authors:  Ignacio Mejía-Calvo; Leslie Muñoz-García; Alexis Jiménez-Uribe; Rosa Camacho-Sandoval; Edith González-González; Gabriela Mellado-Sánchez; Alejandra V Tenorio-Calvo; Carlos A López-Morales; Marco A Velasco-Velázquez; Lenin Pavón; Sonia Mayra Pérez-Tapia; Emilio Medina-Rivero
Journal:  Biotechnol Rep (Amst)       Date:  2019-04-18

5.  Preclinical evaluation of a replication-deficient intranasal DeltaNS1 H5N1 influenza vaccine.

Authors:  Julia Romanova; Brigitte M Krenn; Markus Wolschek; Boris Ferko; Ekaterina Romanovskaja-Romanko; Alexander Morokutti; Anna-Polina Shurygina; Sabine Nakowitsch; Tanja Ruthsatz; Bettina Kiefmann; Ulrich König; Michael Bergmann; Monika Sachet; Shobana Balasingam; Alexander Mann; John Oxford; Martin Slais; Oleg Kiselev; Thomas Muster; Andrej Egorov
Journal:  PLoS One       Date:  2009-06-19       Impact factor: 3.240

6.  HIV-1 activates macrophages independent of Toll-like receptors.

Authors:  Joseph N Brown; James J Kohler; Carter R Coberley; John W Sleasman; Maureen M Goodenow
Journal:  PLoS One       Date:  2008-12-02       Impact factor: 3.240
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.