Literature DB >> 1366593

Rapid in vitro transformation system for liver epithelial cells by iron chelate, Fe-NTA.

M Yamada1, M Awai, T Okigaki.   

Abstract

We have previously found toxic effects of iron chelate, Fe-NTA on cultured normal rat liver epithelial cells (RL34). In the present study, when RL34 cells were exposed to 50 micrograms/ml iron of Fe-NTA for 15 days, besides the expected cytolytic effects in most cells, the appearance of resistant cells was observed. The resistant cells showed drastic morphological transformation, grew in soft agar, and induced hepatocellular carcinomas when transplanted into syngeneic newborn rats in a short period of time. Since DNA instability in the transformed cells was ascertained by differential AO staining, it is suggested that DNA damage by Fe-NTA is of a critical importance for extremely rapid neoplastic transformation of normal epithelial cells.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 1366593     DOI： 10.1007/bf00143677

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  20 in total

1.  Role of superoxide radicals in cytotoxic effects of Fe-NTA on cultured normal liver epithelial cells.

Authors:  M Yamada; T Okigaki; M Awai
Journal:  Cell Struct Funct       Date:  1987-10       Impact factor: 2.212

Review 2.  Oxygen free radicals and iron in relation to biology and medicine: some problems and concepts.

Authors:  B Halliwell; J M Gutteridge
Journal:  Arch Biochem Biophys       Date:  1986-05-01       Impact factor: 4.013

3.  Hepatocellular carcinoma in Great Britain: influence of age, sex, HBsAg status, and aetiology of underlying cirrhosis.

Authors:  P J Johnson; N Krasner; B Portmann; A L Eddleston; R Williams
Journal:  Gut       Date:  1978-11       Impact factor: 23.059

4.  Different instability of nuclear DNA at acid hydrolysis in cancerous and noncancerous cells as revealed by fluorescent staining with acridine orange.

Authors:  M Fukuda; N Miyoshi; T Hattori; H Sugihara; Y Hosokawa; K Nakanishi
Journal:  Histochemistry       Date:  1986

5.  Active oxygen acts as a promoter of transformation in mouse embryo C3H/10T1/2/C18 fibroblasts.

Authors:  R Zimmerman; P Cerutti
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

6.  Mechanism of iron toxicity in the liver and pancreas after a single injection of ferric nitrilotriacetate.

Authors:  Y Yamanoi; R Matsuura; M Awai
Journal:  Nihon Ketsueki Gakkai Zasshi       Date:  1982-12

Review 7.  Iron overload disorders: natural history, pathogenesis, diagnosis, and therapy.

Authors:  G D McLaren; W A Muir; R W Kellermeyer
Journal:  Crit Rev Clin Lab Sci       Date:  1983       Impact factor: 6.250

8.  Induction of diabetes in animals by parenteral administration of ferric nitrilotriacetate. A model of experimental hemochromatosis.

Authors:  M Awai; M Narasaki; Y Yamanoi; S Seno
Journal:  Am J Pathol       Date:  1979-06       Impact factor: 4.307

9.  Lipid peroxidation and cytotoxicity of Ehrlich ascites tumor cells by ferric nitrilotriacetate.

Authors:  S Nakamoto; Y Yamanoi; T Kawabata; Y Sadahira; M Mori; M Awai
Journal:  Biochim Biophys Acta       Date:  1986-10-31

10.  Surface membrane nucleoside triphosphatase activity and tumorigenicity of cultured liver epithelial cells.

Authors:  S Karasaki; T Okigaki
Journal:  Cancer Res       Date:  1976-12       Impact factor: 12.701
View more
  4 in total

Review 1.  Iron toxicity and chelation therapy.

Authors:  Robert S Britton; Katherine L Leicester; Bruce R Bacon
Journal:  Int J Hematol       Date:  2002-10       Impact factor: 2.490

2.  Curcumin and Turmeric Modulate the Tumor-Promoting Effects of Iron In Vitro.

Authors:  Donald J Messner; Todd Robinson; Kris V Kowdley
Journal:  Nutr Cancer       Date:  2017-01-27       Impact factor: 2.900

3.  Neoplastic transformation of rat liver epithelial cells is enhanced by non-transferrin-bound iron.

Authors:  Donald J Messner; Kris V Kowdley
Journal:  BMC Gastroenterol       Date:  2008-02-06       Impact factor: 3.067

4.  Perillyl Alcohol Protects Against Fe-NTA-Induced Nephrotoxicity and Early Tumor Promotional Events in Rat Experimental Model.

Authors:  Tamanna Jahangir; Sarwat Sultana
Journal:  Evid Based Complement Alternat Med       Date:  2007-12       Impact factor: 2.629
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.