Literature DB >> 1366587

An improved method for rapid isolation of plasmid DNA from wild-type gram-negative bacteria for plasmid restriction profile analysis.

J E Olsen1.   

Abstract

An improved method for the isolation of large and small plasmids from wild-type Gram-negative bacteria has been developed. The protocol combines the lysis and purification procedures of two popular plasmid isolation methods, and produces DNA sufficiently pure for restriction enzyme digestion in less than three hours.

Mesh：

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Year:  1990        PMID： 1366587     DOI： 10.1111/j.1472-765x.1990.tb01335.x

Source DB:  PubMed          Journal:  Lett Appl Microbiol        ISSN： 0266-8254            Impact factor:   2.858


  17 in total

1.  VIM-12, a novel plasmid-mediated metallo-beta-lactamase from Klebsiella pneumoniae that resembles a VIM-1/VIM-2 hybrid.

Authors:  Spyros Pournaras; Alexandros Ikonomidis; Leonidas S Tzouvelekis; Despoina Tokatlidou; Nicholas Spanakis; Antonios N Maniatis; Nicholas J Legakis; Athanassios Tsakris
Journal:  Antimicrob Agents Chemother       Date:  2005-12       Impact factor: 5.191

2.  Biochemical and molecular characterization of Salmonella enterica serovar berta, and comparison of methods for typing.

Authors:  J E Olsen; D J Brown; D L Baggesen; M Bisgaard
Journal:  Epidemiol Infect       Date:  1992-04       Impact factor: 2.451

3.  Comparison of Pulsed-Field Gel Electrophoresis, Ribotyping, and Plasmid Profiling for Typing of Vibrio anguillarum Serovar O1.

Authors:  M N Skov; K Pedersen; J L Larsen
Journal:  Appl Environ Microbiol       Date:  1995-04       Impact factor: 4.792

4.  Evaluation and comparison of molecular techniques for epidemiological typing of Salmonella enterica subsp. enterica serovar dublin.

Authors:  B Liebisch; S Schwarz
Journal:  J Clin Microbiol       Date:  1996-03       Impact factor: 5.948

5.  Restriction fragment length polymorphism of the Vibrio anguillarum serovar O1 virulence plasmid.

Authors:  J E Olsen; J L Larsen
Journal:  Appl Environ Microbiol       Date:  1990-10       Impact factor: 4.792

6.  Utilization of both phenotypic and molecular analyses to investigate an outbreak of multidrug-resistant Salmonella anatum in horses.

Authors:  F A Hartmann; S E West
Journal:  Can J Vet Res       Date:  1997-07       Impact factor: 1.310

7.  Restriction fragment length polymorphism of the pMJ101-like plasmid and ribotyping in the fish pathogen Vibrio ordalii.

Authors:  K Pedersen; S Koblavi; T Tiainen; P A Grimont
Journal:  Epidemiol Infect       Date:  1996-10       Impact factor: 2.451

8.  Molecular characterization of plasmids in Salmonella enteritidis phage types.

Authors:  D J Brown; E J Threlfall; M D Hampton; B Rowe
Journal:  Epidemiol Infect       Date:  1993-04       Impact factor: 2.451

9.  Heterogeneity among isolates of Vibrio vulnificus recovered from eels (Anguilla anguilla) in Denmark.

Authors:  L Høi; I Dalsgaard; A DePaola; R J Siebeling; A Dalsgaard
Journal:  Appl Environ Microbiol       Date:  1998-12       Impact factor: 4.792

10.  Molecular epidemiology of ampicillin-resistant clinical isolates of Salmonella enteritidis.

Authors:  A C Vatopoulos; E Mainas; E Balis; E J Threlfall; M Kanelopoulou; V Kalapothalki; H Malamou-Lada; N J Legakis
Journal:  J Clin Microbiol       Date:  1994-05       Impact factor: 5.948
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