Literature DB >> 13635021

Selection of auxotrophic bacterial mutants by tritium-labeled thymidine.

M LUBIN.   

Abstract

A method is proposed for the efficient isolation of auxotrophic mutants of cells of diverse origin by the use of radioactive materials. An example is described in which mutants of Escherichia coli B were selected by growing irradiated cells in the presence of tritium-labeled thymidine.

Entities:  

Keywords:  ESCHERICHIA COLI; NUCLEOSIDES AND NUCLEOTIDES

Mesh：

Substances：

Year:  1959        PMID： 13635021     DOI： 10.1126/science.129.3352.838

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  12 in total

1.  Mutant enrichment in the colonial alga, Eudorina elegans.

Authors:  A L Toby; C L Kemp
Journal:  Genetics       Date:  1975-10       Impact factor: 4.562

2.  Studies on induced variation in the rhizobia. I. Defined media and nodulation test techniques.

Authors:  E A SCHWINGHAMER
Journal:  Appl Microbiol       Date:  1960-11

3.  Enrichment of auxotrophic mutant populations by recycling.

Authors:  M LUBIN
Journal:  J Bacteriol       Date:  1962-03       Impact factor: 3.490

4.  Specific enrichment of mutants of Escherichia coli with an altered acyl CoA synthetase by tritium suicide.

Authors:  F F Hill; D Angelmaier
Journal:  Mol Gen Genet       Date:  1972

5.  Use of griseofulvin for the isolation of auxotrophic mutants of Rhodotorula sp.

Authors:  K Nand; R Joseph; T N Rao
Journal:  Experientia       Date:  1973-02-15

6.  Use of thymineless death to enrich for doubly auxotrophic mutants of Bacillus megaterium.

Authors:  J T Wachsman; L Hogg
Journal:  J Bacteriol       Date:  1964-05       Impact factor: 3.490

7.  Mutations affecting transport of the hexitols D-mannitol, D-glucitol, and galactitol in Escherichia coli K-12: isolation and mapping.

Authors:  J Lengeler
Journal:  J Bacteriol       Date:  1975-10       Impact factor: 3.490

8.  Enrichment of auxotrophic mutants of Aspergillus flavus by tritium suicide.

Authors:  J A Donkersloot; R I Mateles
Journal:  J Bacteriol       Date:  1968-11       Impact factor: 3.490

9.  Use of 8-azaguanine for the isolation of auxotrophic mutants of Bacillus megaterium.

Authors:  J T WACHSMAN; R MANGALO
Journal:  J Bacteriol       Date:  1962-01       Impact factor: 3.490

10.  Macromolecular synthesis in newly transformed cells of Bacillus subtilis.

Authors:  C McCarthy; E W Nester
Journal:  J Bacteriol       Date:  1967-07       Impact factor: 3.490
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