Literature DB >> 1363404

More on effects of storage time and temperature on urinary enzymes: a 1-year study.

E Matteucci1, L Pellegrini, C Uncini-Manganelli, M Cecere, M Saviozzi, O Giampietro.   

Abstract

Results of our conclusive study on urinary enzyme stability during sample storage are reported. We measured alanine aminopeptidase (AAP) and N-acetyl-beta-D-glucosaminidase (NAG) in morning urines from 9 healthy normal subjects immediately after collection and throughout a 1-year storage at -70 and -20 degrees C. AAP proved to be quite stable at -70 degrees C (99.2% of the basal value at the end of the year). NAG is partially preserved (84.1% of the basal value) at -70 degrees C, but significantly decreased (50.4%) at -20 degrees C.

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Year:  1992        PMID: 1363404     DOI: 10.1159/000468796

Source DB:  PubMed          Journal:  Enzyme        ISSN: 0013-9432


  2 in total

1.  Effect of storage time at -20°C on markers used for assessment of renal damage in children: albumin, γ-glutamyl transpeptidase, N-acetyl-β-D-glucosaminidase and α1-microglobulin.

Authors:  Felicia Trachtenberg; Lars Barregard
Journal:  Scand J Urol Nephrol       Date:  2010-06-21

2.  The influence of urinary flow rate in children on excretion of markers used for assessment of renal damage: albumin, gamma-glutamyl transpeptidase, N-acetyl-beta-D -glucosaminidase, and alpha1-microglobulin.

Authors:  Felicia Trachtenberg; Lars Barregard; Sonja McKinlay
Journal:  Pediatr Nephrol       Date:  2007-08-18       Impact factor: 3.714

  2 in total

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