| Literature DB >> 1358206 |
Abstract
Photoreceptor guanylyl cyclase activity is modulated by an endogenous calcium-binding protein called recoverin. A modified isolation procedure for recoverin using gel-filtration chromatography instead of a heat denaturation step is presented. The elution volume of recoverin corresponds to a monomer. Recoverin exhibits a calcium-dependent mobility shift in a native gel electrophoresis. Isoelectric focusing revealed a pI of 5.25. No subspecies of recoverin were detected.Entities:
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Year: 1992 PMID: 1358206 DOI: 10.1016/0167-4838(92)90038-f
Source DB: PubMed Journal: Biochim Biophys Acta ISSN: 0006-3002