Literature DB >> 13563543

Intracellular distribution of 5' nucleotidase in rat liver.

G DE LAMIRANDE, C ALLARD, A CANTERO.   

Abstract

The intracellular distribution of 5' nucleotidase was investigated in rat liver by biochemical analysis of cell fractions obtained by differential centrifugation. The enzymatic activity was measured by determination of the inorganic phosphorus liberated from 5' nucleotides. The 5' nucleotidase activity was mainly found in the nuclear and microsomal fractions. An attempt to extract the enzyme from these fractions with Mg(++) ion solutions was unsuccessful. It is concluded that 5' nucleotidase is actually present in the nuclear and microsomal fractions of rat liver cells.

Entities:  

Keywords:  LIVER/metabolism; PHOSPHATASES

Mesh:

Substances:

Year:  1958        PMID: 13563543      PMCID: PMC2224501          DOI: 10.1083/jcb.4.4.373

Source DB:  PubMed          Journal:  J Biophys Biochem Cytol        ISSN: 0095-9901


  7 in total

1.  Influence of cations on the intracellular distribution of rat liver arginase.

Authors:  J D GORRY; B GOTTLIEB; O ROSENTHAL; H M VARS
Journal:  J Biol Chem       Date:  1956-11       Impact factor: 5.157

2.  Histochemical studies of 5 nucleotidase. I. Method and specificity.

Authors:  J F A MCMANUS; C H LUPTON; G HARDEN
Journal:  Lab Invest       Date:  1952       Impact factor: 5.662

3.  Alkaline phosphatase substrate specificities in cultured normal and malignant cells of mouse, rat, and fowl.

Authors:  J J BIESELE; A Y WILSON
Journal:  Cancer Res       Date:  1951-03       Impact factor: 12.701

4.  Further studies on the histochemical specificity of phosphatases.

Authors:  G GOMORI
Journal:  Proc Soc Exp Biol Med       Date:  1949-11

5.  Mitochondrial population in mammalian cells. I. Description of a counting technic and preliminary results on rat liver in different physiological and pathological conditions.

Authors:  C ALLARD; R MATHIEU; G DE LAMIRANDE; A CANTERO
Journal:  Cancer Res       Date:  1952-06       Impact factor: 12.701

6.  Histochemical Demonstration of 5-Nucleotidase Activity in Cell Nuclei.

Authors:  M Wachstein; E Meisel
Journal:  Science       Date:  1952-06-13       Impact factor: 47.728

7.  Histochemical studies of 5-nucleotidase. II. 5-Nucleotidase in glomerular obsolescence.

Authors:  J F A MCMANUS; C H LUPTON
Journal:  Lab Invest       Date:  1953 Jan-Feb       Impact factor: 5.662

  7 in total
  4 in total

1.  Rat liver 5'-nucleotidase.

Authors:  A A el-Aaser; E Reid
Journal:  Histochem J       Date:  1969-08

2.  5'-nucleotidase. I. Distribution of 5'-nucleotidase in tissues of rat and mouse.

Authors:  M J Hardonk
Journal:  Histochemie       Date:  1968

3.  Isolation and characterization of the urothelial lumenal plasma membrane.

Authors:  J S Caruthers; M A Bonneville
Journal:  J Cell Biol       Date:  1977-05       Impact factor: 10.539

4.  Analytical study of microsomes and isolated subcellular membranes from rat liver. II. Preparation and composition of the microsomal fraction.

Authors:  A Amar-Costesec; H Beaufay; M Wibo; D Thinès-Sempoux; E Feytmans; M Robbi; J Berthet
Journal:  J Cell Biol       Date:  1974-04       Impact factor: 10.539

  4 in total

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