Literature DB >> 1353905

Calcium-dependent transmitter secretion reconstituted in Xenopus oocytes: requirement for synaptophysin.

J Alder1, B Lu, F Valtorta, P Greengard, M M Poo.   

Abstract

Calcium-dependent glutamate secretion was reconstituted in Xenopus oocytes by injecting the oocyte with total rat cerebellar messenger RNA (mRNA). Co-injection of total mRNA with antisense oligonucleotides to synaptophysin message decreased the expression of synaptophysin in the oocyte and reduced the calcium-dependent secretion. A similar effect on secretion was observed for oocytes injected with total mRNA together with an antibody to rat synaptophysin. These results indicate that synaptophysin is necessary for transmitter secretion and that the oocyte expression system may be useful for dissecting the molecular events associated with the secretory process.

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Year:  1992        PMID： 1353905     DOI： 10.1126/science.1353905

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  32 in total

1.  Synaptophysins: vesicular cation channels?

Authors:  Oussama El Far; Heinrich Betz
Journal:  J Physiol       Date:  2002-03-01       Impact factor: 5.182

2.  Ca(2+)-regulated, neurosecretory granule channel involved in release from neurohypophysial terminals.

Authors:  Yong Yin; Govindan Dayanithi; José R Lemos
Journal:  J Physiol       Date:  2002-03-01       Impact factor: 5.182

3.  Molecular identification and reconstitution of depolarization-induced exocytosis monitored by membrane capacitance.

Authors:  Roy Cohen; Bernhard M Schmitt; Daphne Atlas
Journal:  Biophys J       Date:  2005-09-08       Impact factor: 4.033

Review 4.  Acetylcholine release and the cholinergic genomic locus.

Authors:  M Israël; Y Dunant
Journal:  Mol Neurobiol       Date:  1998-02       Impact factor: 5.590

Review 5.  In vitro reconstitution of neurotransmitter release.

Authors:  Y Dunant; M Israël
Journal:  Neurochem Res       Date:  1998-05       Impact factor: 3.996

Review 6.  From oocyte to neuron: do neurotransmitters function in the same way throughout development?

Authors:  G A Buznikov; Y B Shmukler; J M Lauder
Journal:  Cell Mol Neurobiol       Date:  1996-10       Impact factor: 5.046

7.  Second messengers, trafficking-related proteins, and amino acid residues that contribute to the functional regulation of the rat brain GABA transporter GAT1.

Authors:  M W Quick; J L Corey; N Davidson; H A Lester
Journal:  J Neurosci       Date:  1997-05-01       Impact factor: 6.167

8.  Constitutive secretion of exogenous neurotransmitter by nonneuronal cells: implications for neuronal secretion.

Authors:  S Chang; R Girod; T Morimoto; M O'Donoghue; S Popov
Journal:  Biophys J       Date:  1998-09       Impact factor: 4.033

9.  Metabotropic glutamate receptors activate G-protein-coupled inwardly rectifying potassium channels in Xenopus oocytes.

Authors:  J A Saugstad; T P Segerson; G L Westbrook
Journal:  J Neurosci       Date:  1996-10-01       Impact factor: 6.167

10.  Synaptophysin, a major synaptic vesicle protein, is not essential for neurotransmitter release.

Authors:  H T McMahon; V Y Bolshakov; R Janz; R E Hammer; S A Siegelbaum; T C Südhof
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-14       Impact factor: 11.205
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