Literature DB >> 1349882

Immunogold labelling is a quantitative method as demonstrated by studies on aminopeptidase N in microvillar membrane vesicles.

G H Hansen1, L L Wetterberg, H Sjöström, O Norén.   

Abstract

Microvillar membrane vesicle preparations with varying content of aminopeptidase N were prepared from enterocytes of the pig small intestine. Postembedding immunogold labelling of aminopeptidase N was performed on these glutaraldehyde/paraformaldehyde-fixed, osmium tetroxide-treated and Epon-embedded microvillar membrane vesicles. The number of gold particles per micrometre microvillar membrane (labelling intensity) was calculated and compared to the corresponding enzymatic activity. A very close relationship was found between labelling intensity and aminopeptidase N activity, demonstrating that postembedding immunogold labelling can be used quantitatively.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1349882     DOI： 10.1007/bf01047462

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  13 in total

Review 1.  Simultaneous demonstration of multiple antigens by indirect immunofluorescence or immunogold staining. Novel light and electron microscopical double and triple staining method employing primary antibodies from the same species.

Authors:  B L Wang; L I Larsson
Journal:  Histochemistry       Date:  1985

2.  One antigen, one gold? A quantitative analysis of immunogold labeling of plasma membrane 5'-nucleotidase in frozen thin sections.

Authors:  K E Howell; U Reuter-Carlson; E Devaney; J P Luzio; S D Fuller
Journal:  Eur J Cell Biol       Date:  1987-10       Impact factor: 4.492

3.  Postembedding immunogold labelling of fixed glutamate: an electron microscopic analysis of the relationship between gold particle density and antigen concentration.

Authors:  O P Ottersen
Journal:  J Chem Neuroanat       Date:  1989 Jan-Feb       Impact factor: 3.052

4.  An immunocolloid method for the electron microscope.

Authors:  W P Faulk; G M Taylor
Journal:  Immunochemistry       Date:  1971-11

5.  Quantitation in immunocytochemistry: correlation of immunogold labeling to absolute number of membrane antigens.

Authors:  G Griffiths; H Hoppeler
Journal:  J Histochem Cytochem       Date:  1986-11       Impact factor: 2.479

6.  Immunomicroscopic localization of aminopeptidase N in the pig enterocyte. Implications for the route of intracellular transport.

Authors:  G H Hansen; H Sjöström; O Norén; E Dabelsteen
Journal:  Eur J Cell Biol       Date:  1987-04       Impact factor: 4.492

7.  A new method of preparing gold probes for multiple-labeling cytochemistry.

Authors:  J W Slot; H J Geuze
Journal:  Eur J Cell Biol       Date:  1985-07       Impact factor: 4.492

8.  Organelles involved in the intracellular transport of newly synthesized aminopeptidase N and their acidity.

Authors:  G H Hansen; E M Danielsen; H Sjöström; O Norén
Journal:  Eur J Cell Biol       Date:  1989-06       Impact factor: 4.492

9.  Usefulness of the immunogold technique in quantitation of a soluble protein in ultra-thin sections.

Authors:  G Posthuma; J W Slot; H J Geuze
Journal:  J Histochem Cytochem       Date:  1987-04       Impact factor: 2.479

10.  The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.

Authors:  E S REYNOLDS
Journal:  J Cell Biol       Date:  1963-04       Impact factor: 10.539
View more
  10 in total

1.  Comparison of different detection methods in quantitative microdensitometry.

Authors:  L Ermert; A C Hocke; H R Duncker; W Seeger; M Ermert
Journal:  Am J Pathol       Date:  2001-02       Impact factor: 4.307

2.  Deep-apical tubules: dynamic lipid-raft microdomains in the brush-border region of enterocytes.

Authors:  Gert H Hansen; Jens Pedersen; Lise-Lotte Niels-Christiansen; Lissi Immerdal; E Michael Danielsen
Journal:  Biochem J       Date:  2003-07-01       Impact factor: 3.857

3.  IgG trafficking in the adult pig small intestine: one- or bidirectional transfer across the enterocyte brush border?

Authors:  Rebecca Möller; Gert H Hansen; E Michael Danielsen
Journal:  Histochem Cell Biol       Date:  2016-09-19       Impact factor: 4.304

4.  Caveolae/lipid rafts in fibroblast-like synoviocytes: ectopeptidase-rich membrane microdomains.

Authors:  D Riemann; G H Hansen; L Niels-Christiansen; E Thorsen; L Immerdal; A N Santos; A Kehlen; J Langner; E M Danielsen
Journal:  Biochem J       Date:  2001-02-15       Impact factor: 3.857

5.  The coronavirus transmissible gastroenteritis virus causes infection after receptor-mediated endocytosis and acid-dependent fusion with an intracellular compartment.

Authors:  G H Hansen; B Delmas; L Besnardeau; L K Vogel; H Laude; H Sjöström; O Norén
Journal:  J Virol       Date:  1998-01       Impact factor: 5.103

6.  Scavenger receptor class B type I (SR-BI) in pig enterocytes: trafficking from the brush border to lipid droplets during fat absorption.

Authors:  G H Hansen; L-L Niels-Christiansen; L Immerdal; E M Danielsen
Journal:  Gut       Date:  2003-10       Impact factor: 23.059

7.  Galectin-4 and small intestinal brush border enzymes form clusters.

Authors:  E M Danielsen; B van Deurs
Journal:  Mol Biol Cell       Date:  1997-11       Impact factor: 4.138

8.  C4.4A gene ablation is compatible with normal epidermal development and causes modest overt phenotypes.

Authors:  Mette Camilla Kriegbaum; Benedikte Jacobsen; Annette Füchtbauer; Gert Helge Hansen; Ib Jarle Christensen; Carsten Friis Rundsten; Morten Persson; Lars Henning Engelholm; Andreas Nygaard Madsen; Ivano Di Meo; Ida Katrine Lund; Birgitte Holst; Andreas Kjaer; Ole Didrik Lærum; Ernst-Martin Füchtbauer; Michael Ploug
Journal:  Sci Rep       Date:  2016-05-12       Impact factor: 4.379

9.  Apical secretion of apolipoproteins from enterocytes.

Authors:  E M Danielsen; G H Hansen; M D Poulsen
Journal:  J Cell Biol       Date:  1993-03       Impact factor: 10.539

10.  Caveolae in fibroblast-like synoviocytes: static structures associated with vimentin-based intermediate filaments.

Authors:  Kasper D Berg; Raluca M Tamas; Anne Riemann; Lise-Lotte Niels-Christiansen; Gert H Hansen; E Michael Danielsen
Journal:  Histochem Cell Biol       Date:  2008-07-22       Impact factor: 4.304
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.