J P Burnie1, A Coke, R C Matthews. 1. Department of Medical Microbiology, University of Manchester Medical School.
Abstract
AIMS: To develop a genome based DNA fingerprinting system for Aspergillus fumigatus mould. METHODS: DNA was extracted from 21 isolates obtained from eight patients with an aspergilloma. This was with a freeze-dried mycelial extract fragmented in liquid nitrogen. DNA was subsequently purified by phenol-chloroform extraction followed by ultracentrifugation on a caesium chloride gradient. The DNA was restricted by EcoRI and Xba I. RESULTS: All isolates were identical when cut by EcoRI; Xba I delineated six DNA types. CONCLUSIONS: DNA fingerprinting can be used to type isolates of A fumigatus. Strains from within an aspergilloma which were morphologically distinct could either have the identical DNA fingerprint or produce a unique type.
AIMS: To develop a genome based DNA fingerprinting system for Aspergillus fumigatus mould. METHODS: DNA was extracted from 21 isolates obtained from eight patients with an aspergilloma. This was with a freeze-dried mycelial extract fragmented in liquid nitrogen. DNA was subsequently purified by phenol-chloroform extraction followed by ultracentrifugation on a caesium chloride gradient. The DNA was restricted by EcoRI and Xba I. RESULTS: All isolates were identical when cut by EcoRI; Xba I delineated six DNA types. CONCLUSIONS: DNA fingerprinting can be used to type isolates of A fumigatus. Strains from within an aspergilloma which were morphologically distinct could either have the identical DNA fingerprint or produce a unique type.
Authors: E Rodriguez; T De Meeüs; M Mallie; F Renaud; F Symoens; P Mondon; M A Piens; B Lebeau; M A Viviani; R Grillot; N Nolard; F Chapuis; A M Tortorano; J M Bastide Journal: J Clin Microbiol Date: 1996-10 Impact factor: 5.948
Authors: N Vanittanakom; C R Cooper; S Chariyalertsak; S Youngchim; K E Nelson; T Sirisanthana Journal: J Clin Microbiol Date: 1996-07 Impact factor: 5.948